摘要
本试验选取334只天府肉羊、波尔山羊和成都麻羊为试验材料,对山羊FSHβ基因进行了PCR扩增,采用PCR-SSCP和SNP技术分析了该基因在山羊中的多态性。结果表明,试验羊FSHβ基因5′调控区存在第712位A→G突变,形成了AA、AB和BB 3种基因型。适合性检验和最小二乘分析结果显示,山羊FSHβ基因不同基因型的平均窝产羔数存在显著差异;天府肉羊和成都麻羊的FSHβ多态位点处于Hardy-Weinberg平衡状态(P>0.05),波尔山羊极显著偏离了Hardy-Weinberg平衡(P<0.01)。由此推测,FSHβ基因可能对于山羊繁殖性能具有较大影响或与控制繁殖性能的主基因连锁,可以尝试将其作为一个重要的分子标记用于山羊的育种实践。
In this study,334 Tianfu goats,Boer and Chengdu Ma goats were used to analyze FSHβ gene polymorphism by PCR-SSCP.Results indicated that there was one mutation site in the 5′regulatory region of FSHβ gene among three types of the goat,that was nucleobase A in place of nucleobase G in 712 site.The Chi-square test for goodness of fit showed that the frequencies of the FSHβ genotypes in the population of Tianfu goats and Chengdu Ma goats agreed with Hardy-Weinberg equilibrium（P〉0.05）,while the Boer goat significantly deviated it（P〈0.01）.The results indicated that the mutation in the 5′regulatory region significantly affected the litter sizes of goat,therefore,it could be preliminarily deduced that FSHβ gene was probably a major gene or a QTL linked gene which associated with reproduction traits in goat.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第4期150-153,共4页
China Animal Husbandry & Veterinary Medicine
基金
国家现代肉羊产业技术体系(NYCYTX-39)
四川省"十一五"畜禽育种攻关项目(01N9002-06)
