摘要
利用RAPD分子标记对35个短枝木麻黄(Casuarina equisefi folia)种源的遗传多样性和亲缘关系进行了分析.15个随机引物共扩增了出201条DNA带,其中148条为多态性带,占73.63%,平均每个引物扩增出9.9条多态性带.种源的Shannon's多样性指数为0.435 4.Nei's基因多样度为0.288 4,表明短枝木麻黄种源的遗传多样性水平较高.35个种源彼此的遗传距离在0.088 4～0.753 9之间,平均0.404 5,经过UPGMA聚类分析将35个种源划分为A、B、C、D、E、F 6个类群,其中A群5个种源,B群17个种源.C群4个种源.D群2个种源.E群1个种源,F群6个种源.
Genetic diversity and phylogenetic relationship of 35 Casuarina equisetifolia provenances were studied by RAPD marker. 201 bands were identified with 15 oligonucleotide primers,out of which 145 bands were polymorphic and accounted for 73.63%. The average number of DNA bands amplified by each primer was 9.9. The average Shannon＇s index of diversity and Nei＇s gene diversity were 0. 435 4 and 0. 288 4 respectively. The results showed that there was great genetic diversity within C. equisetifolia provenances,which provided base for genetic selection and breeding. The genetic distance among 35 provenances ranged 0. 088 4 to 0. 753 9 and the average was 0. 404 5. Based on UPGMA cluster analysis, 35 provenances were divided into 6 groups .. group A,B,C, D, E and F. Group A included 5 provenances, group B included 17 provenances, group C included 4 provenances, group D included 2 provenances,group E included 1 provenances and group F included 17 provenances. They showed close phylogenetic relationship within groups respectively.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第6期856-860,共5页
Journal of Xiamen University：Natural Science
基金
国家"十五"攻关项目(2004BA516AB-15)
国家948项目(96-4-32)
福建省林木种苗攻关项目资助
关键词
短枝木麻黄
种源
遗传多样性
RAPD
Casuarina equisetifolia
provenance
genetic diversity
RAPD
