摘要
目的:建立茶色素注射液中没食子酸含量的测定方法。方法:采用反相高效液相色谱法,色谱柱为C18,流动相为乙酸-甲 醇-二甲基甲酰胺-水(2:8:30:160),检测波长为280nm,流速为0．5ml／min,柱温为20℃。结果:没食子酸检测浓度在8．4- 92．4μg／ml范围内线性关系良好(r=0．9 997),平均加样回收率为99．13％(RSD=1．55％)。结论:本方法特异性、灵敏度、精密 度、回收率高,重现性好,可为茶色素注射液质量标准的制订提供依据。
OBJECTIVE： To develop a method for the determination of gallic acid in tea pigment injection. METHODS： RP - HLPC method was adopted in which the separation of gallic acid was performed on C18 chromatographic column with a column temperature of 20℃ ; the mobile phase was composed of acetic acid -MeOH -DMF -H2O（2 ： 8 ： 30 ： 160） with a flow rate of 0.5ml/min and detection wavelength of 280nm.RESULTS： The linear range of gallic acid was 8.4-92.4μg/ml（r = 0.9 997）, the average recovery rate was 99.13% （RSD = 1.55% ） .CONCLUSION：The method developed in the present study is of high specificity, sensitivity, precision, recovery and reproducibility and it can provide a basis for the formulation of quality comrol method for tea pigment injection.
出处
《中国药房》
CAS
CSCD
北大核心
2005年第21期1657-1658,共2页
China Pharmacy
