摘要
目的:探讨实时荧光定量PCR(FQ-PCR)检测HBV-DNA在乙型肝炎病毒(HBV)感染的诊疗中的应用价值。方法:用实时荧光定量PCR(FQ-PCR)方法定量检测523例患者的血清HBV-DNA含量(临界值为500cop ies/m l),同时用ELISA法测定HBV血清标记物。结果:经FQ-PCR检测,116份HbsAg,HbeAg,HbcAb,ProS1-Ag都阳性的标本,HBV-DNA阳性率为98.7%,平均拷贝数为1.87×108copy/m l,显著高于其他组(P<0.05)。HbsAg,HbeAg,ProS1-Ag一项或多项阳性者的HBV-DNA阳性率和平均拷贝数显高于三项抗原全阴者。结论:FQ-PCR可以快速而准确地检测HBV-DNA拷贝数。HBV-DNA可准确、灵敏地反映HBV复制。与HBV血清标记物联合应用可使诊断更准确,治疗更合理。
Objective：To explore the value of HBV-DNA copies detected by real-time fluorescence quantitative PCR （FQ- PCR） in the diagnosis and therapy of HBV infection. Methods：523 patients＇serum HBV-DNA were detected by real-time FQ- PCR. The cutoff is 500 copies/ml. Their serological markers of HBV were detected by ELISA method. Both results of HBV-DNA and serological markers of HBV were compared. Results： In the 116 HbsAg （ ＋ ）, HbeAg （ ＋ ） , HbcAb （ ＋ ） and ProS1-Ag （ ＋ ） samples, HBV-DNA positive ratio and mean of copies are 98.7% and 1.87 × 10^8copies/ml. They are much higher than that of other greups（P 〈0.05）. The HbsAg （-）, HbeAg （-）, ProS1-Ag （-） group＇s positive ratio and copy number of HBV- DNA are much lower than that of HbsAg （ ＋ ） and/or HbeAg （ ＋ ）, and/or ProS1-Ag （ ＋ ） group （ P 〈 0.05）. Conclusion： FQ-PCR is a fast and accurate technique to quantitative the serum HBV-DNA. HBV-DNA can reflect HBV replication accurately. Combination the HBV-DNA and the serological markers of HBV would make the diagnosis and therapy of HBV infection more reasonable and efficient.
出处
《军医进修学院学报》
CAS
北大核心
2005年第5期344-345,共2页
Academic Journal of Pla Postgraduate Medical School
