摘要
目的评价应用国产试剂的实时荧光定量聚合酶链反应(PCR)在检测乙型肝炎病毒(HBV)DNA中的临床应用。方法基于TaqMan探针技术的实时荧光定量PCR应用Roche公司LightCycler仪器和国产达安试剂,PCR酶联免疫吸附试验(ELISA)应用Roche公司COBASAmplicor全自动内标法系统,用2种方法检测乙型肝炎患者血清中的HBVDNA,评价其灵敏度、精密度及相关性。结果实时荧光定量PCR方法的灵敏度略低于PCR ELISA全自动内标法,两者变异系数(CV)均较低,用2种方法检测临床标本,相关系数为0.890。结论应用国产试剂配合LightCycler仪器的实时荧光定量PCR具有较高的灵敏度和较好的重复性,并与PCR ELISA全自动内标法有良好的相关性。
Objective To evaluate the real-time fluorescent polymerase chain reaction(PCR) quantitative test for hepatitis B virus (HBV) DNA. Methods Real-time PCR and automated quantitative PCR(COBAS Amplicor) were used to detect HBV DNA in serum of hepatitis B patients. The sensitivity intra-assay variability and correlation of the two methods were evaluated. Results The sensitivity of the automated quantitative PCR was slightly higher than that of the real-time fluorescent method. In the case of middle titer of HBV DNA, the CV of the two methods was low. The correlation coefficient between two methods was 0.890. Conclusions Real-time fluorescent quantitative method has high sensitivity and low CV, and has good correlation with COBAS Amplicor in clinical trial.
出处
《检验医学》
CAS
北大核心
2005年第4期319-321,共3页
Laboratory Medicine
