摘要
目的观察尾吊模拟失重后大鼠比目鱼肌中ERK1/2磷酸化状态的变化。方法采用尾部悬吊大鼠模拟失重效应,以Westernblot技术检测游离的大鼠比目鱼肌中总的ERK1/2含量和其磷酸化状态的变化。结果7d和14d尾吊模拟失重后,大鼠比目鱼肌内总ERK1/2的含量没有发生改变。而ERK1磷酸化状态在7d和14d模拟失重后均显著降低。ERK2的磷酸化状态在尾吊7d后显著下降,而在14d尾吊后其下降程度却未达显著性。结论尾吊模拟失重后可降低大鼠比目鱼肌内ERK1/2的磷酸化状态。
Objective To study the changes of ERK1/2 phosphorylation status induced by unloading of soleus muscle after tail suspension in rats. Method Hindlimb unloading（HU） was produced by tail suspension, which simulated the effect of weightlessness on male Wistar rats. Western blot tech- nique was introduced to detect the total and phospho-ERK1/2 status in isolated soleus muscle of 7 d, 14 d HU and repective control groups. Result Total ERK1/2 in soleus muscle was not affected by ？ d and 14 d HU. The ERK1 phosphorylation status were declined significantly after 7 d and 14 d HU. The ERK2 phosphorylation status were declined significantly after 7 d HU, but not for 14 d HU. Conclusion The unloading of soleus muscle after simulated microgravity can decrease the phospho- rylation status of ERK1/2, which may contribute to the atrophy of soleus muscle induced by simulated weightlessness.
出处
《航天医学与医学工程》
CAS
CSCD
北大核心
2005年第5期313-317,共5页
Space Medicine & Medical Engineering
基金
China'sMannedSpaceFlightProject
关键词
失重模拟
尾吊
比目鱼肌
细胞外信号调节激酶
丝裂原活化蛋白激酶
weightlessness simulation
tail suspension
soleus muscle
extracellular signal-regulated kinase
mitogen-activated protein kinase
