摘要
目的观察去甲肾上腺素(NE)对烧伤大鼠脑组织血管内皮细胞生长因子(VEGF)表达的影响。方法用40%总体表面积(TBSA)Ⅲ度烫伤大鼠,观察NE对烧伤大鼠血脑屏障通透性的影响。用免疫组织化学法、实时荧光定量聚合酶链反应(PCR)法,观察NE对烧伤大鼠脑组织VEGF表达的影响。结果(1)烧伤大鼠和NE刺激后的烧伤大鼠血脑屏障通透性增加,与对照组比较差异有统计学意义(P<0.01)。随着烧伤前注入NE剂量的加大,BN2、BN3组脑组织的血脑屏障通透性增加明显,与烧伤组比较,差异有统计学意义(P<0.01);(2)大剂量NE刺激时,脑组织VEGF的表达增加,烧伤后VEGF的表达明显增加,与对照、N1、N2组比较,差异有统计学意义(P<0.01)。随NE刺激剂量增大,烧伤后VEGF表达增强,BN2、BN3组与烧伤组比较,差异有统计学意义(P<0.05)。结论NE能够诱导严重烧伤大鼠脑组织VEGF表达,可能是烧伤后促进脑水肿形成的一个重要因素。
Objective To investigate the effects of norepinephrine （NE） on vascular endothelial growth factor （VEGF） expression of brain tissues in severe burn rats. Methods The healthy male Wistar rats were made into 40%TBSAⅢ°burn models to observe the effect of NE on blood brain barrier. In the meantime, effect of NE was examined by means of immunocytochemistry and real time PCR. Results （ 1 ） Permeability of blood brain barrier was increased in burn and burn with NE stimulating rats, with significantly statistical difference compared with normal control group （ P 〈 0.01 ）. Permeability of blood brain barrier was significantly increased in BN2 and BN3 groups with larger dose norepinephrine stimulation, with significantly statistical difference compared with burn group （ P 〈 0.01 ）. （ 2 ） VEGF expression in the rat brain tissues was increased by larger dose of NE stimulation and increased signifi- candy postburn, with significantly statistical difference compared with normal control, N1 and N2 groups （ P 〈 0.01 ）. VEGF expression was significantly increased post burn with larger dose of NE stimulation in BN2 and BN3 groups, with significantly statistical difference compared with burn group （ P 〈 0.05 ）. Conclusion NE can induce brain tissue VEGF expression in severe burn rats, which may be an important factor for postburn stress response inducing brain edema.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2005年第10期741-744,共4页
Chinese Journal of Trauma
