摘要
研究了１１例正常人及１４例严重脑外伤患者的外周血淋巴细胞糖皮质激素受体水平及血浆皮质醇水平。结果：正常对照组糖皮质激素受体（ＧＣＲ）为６５６１±１６６０位点／细胞，皮质醇（８Ａｍ）为２０８．９±５４．７ｎｇ／ｍｌ；高受体组患者ＧＣＲ为５２５９±１６８６．３位点／细胞，皮质醇（８Ａｍ）为８８０．４±４６９，３ｎｇ／ｍｌ；低受体组患者ＧＣＲ为３００４±１００５．６位点／细胞，皮质醇为１５６５．１±６３１．８ｎｇ／ｍｌ。高受体组患者４例均痊愈，低受体组患者１０例中死亡８例，两组死亡率差异有显著性（Ｐ＜０．０１）。提示，ＧＣＲ与脑外伤患者临床应激状态及糖皮质激素的疗效，预后密切相关。利用外周血淋巴细胞代替脑组织活检用于ＧＣＲ检测具有取材方便、可反复观察、易为患者接受等优点，可反映脑组织ＧＣＲ的变化趋势，有临床应用价值。
Glucocorticoid receptors(GCR)of pheripheral lymphocytes from 14 patients with severe brain injury and 11 normal volunteers were studied, using single point method of radioligand binding assay. All these patients received surgical therapy and glucocorticoid of routine dosage. The results showed that ( 1 ) GCR level are 6561±1660 sites/cell, plasma cortisol (8Am) are 208.9±54.7ng/ml in 11 normal volunteers; ( 2 ) GCR level are 5259± 1686.3 sites/cell, plasma cortisol ( 8Am ) are 880.4 ±469.3ng/ml in 4 patients with GCR>4000 sites/cell, all 4 patients recovered; ( 3 ) GCR level are 3004± 1005.6 sites/cell, plasma cortisol are 1565. 1 ± 631.8ng/ml in 10 patients with GCR<4000 sites/cell,8 patients died.It is indicated that the GCR level can reflect the degree of stress of these patients and their response to glucocorticoid therpy. Using peripheral lymphocytes instead of the brain biopsy for the measurement of GCR can reflect the GCR changes of brain tissue, it is more convenient to get the sample and more acceptable to the patients.
出处
《山东医科大学学报》
1994年第2期114-117,共4页
Acta Academiae Medicinae Shandong
关键词
脑损伤
血液
淋巴细胞
受体
GCR
Brain injuries
Blood
Lymphocytes
Receptors, Glucocorticoid
Adrenal cortex hormones
