摘要
通过向受污湿地土样中添加菌株,探讨微生物对石油烃的降解.实验发现,混合菌能够充分发挥各菌种之间的协同作用,比单菌降解更为有效.最适降解条件控制在pH8.0,温度25℃.适宜的表面活性剂对微生物繁殖进而对油降解具有促进作用,加入300mg.kg-1TW-80降解8d后,菌落数增加了6.22倍,油的降解率提高了20.1%.适量的H2O2有助于细菌分解油,过量会造成菌株死亡,每次添加计量控制在400mg.L-1为宜.经过11d,H2O2累计量达到7000mg.L-1,土样中油的降解率由39.81%提高到66.79%.
To investigate the effect of microbes on oil degradation, we use the method of introducing selected bacteria to the soil samples. Experiment shows that the combined bacteria are preferable to the single ones because of their synergetic functions. The optimum condition for oil degradation was controlled at pH 8.0, 25℃. Some surfactants are helpful to breed bacteria, thus improves the oil degradation. After adding TW-80 （300 mg·kg^-1） for 8 days, the number of bacteria was amplified 6.22 times and the oil degradation rate was enlarged 20.1% . Adequate amount of H2O2 is beneficial for microbes to decompose oil, but its overdose may slay bacteria. 400 mg·L^-1 H2O2 each time is suitable dose. In 11 days after 7 000 mg·L^-1 H2O; was added entirely, the oil degradation rate rose greatly from 39.81% （without H2O2） up to 66.79% .
出处
《环境科学》
EI
CAS
CSCD
北大核心
2005年第5期143-146,共4页
Environmental Science
基金
国家高技术研究发展计划(863)项目(2002AA648010)
关键词
湿地
微生物修复
油
降解
wetland
microbio-remediation
oil
degradation
