摘要
目的:研究鼠李糖脂对微生物降解石油烃废水的影响。方法:通过测定生物量和观察菌株表面来研究鼠李糖脂对菌株的影响;通过正交实验设计,确定石油烃降解率影响因素。通过石油烃降解率的测定,探讨鼠李糖脂与H2O2深度氧化协同作用对微生物降解石油烃的影响。结果:菌株对石油烃的降解率达53%,在相同条件下,添加鼠李糖脂的石油烃降解率提高了12%-20%。添加鼠李糖脂后菌株的生物量明显增多,菌株细胞表面疏水。正交设计表明,影响石油烃降解的主导因子是培养温度,其次是培养时间和鼠李糖脂的添加量。正交设计得到最佳组合为A3B2C1,即培养时间为7d,温度为35℃,鼠李糖脂浓度为60mg/L。3个因素的最佳组合下,石油烃降解率为82%。加入200 mg/L的H2O2时,降解率从82%提高到97%。结论:鼠李糖脂能促进菌株的生长。鼠李糖脂与H2O2深度氧化协同作用有助于微生物对石油烃类污染物降解效率的提高。
Objective： Microbial degradation of petroleum hydrocarbon was studied. Method： By determinating biomass and observating cell surface, the effect of rhanmohp on the strain was studied. By designing orthogonal tests, effective factors of degradation rate was determined. Through degradation rate of petroleum hydrocarbon detemfinated, synergistic effect of rhamnolip and deep oxidation in the microbial degradation of petrohum hydrocarbons was discussed. Result： The degradation rate of diesel oil by the strain reached 53 %. The degradation rate of petroleum hydrocarbon was significantly enhanced by the amcndment of rhamnolipid biosuffaetant. The degradation rate of petroleum hydrocarbon was 12%20% higher than that without rhamnolipid. It indicated that rhamnolipid increased microbial biodegradation and cell surface hydrophobieity. The orthogo- nal tests showed that culture time was the most important factor, followed by the culture time and rhamnolipid concentration. Orthogonal design showed that the best combination was A3 B2 C1 , Which was culture time of seven days, culture temperature of 35 ℃ and rhamnolipid concentration of 60 mg/L. With three of the best combination of factors, the degradation rate of petroletml hydrocarbon was 82%. Biodegradation rate of petroleum hydrocarbon was increased from 82% to 97% by a hydrogen peroxide dosage of 200 mg/L. Conclusion： Rhamnolipid promoted the growth of the strains.Synergistic effect of rhamnolip and deep oxidation would be helpful to increase the biodegradation rate of petroleum hydrocarbon contaminants.
出处
《生物技术》
CAS
CSCD
北大核心
2009年第4期78-81,共4页
Biotechnology
基金
国家自然科学基金项目(30770568)资助
关键词
鼠李糖脂
石油烃
微生物降解
rhanmolip
petroleum hydrocarbon
microbial degradation
