摘要
目的:检测维生素E琥珀酸酯(VES)联合他莫昔芬(tamoxifen)对乳腺癌细胞增殖的抑制作用,并分析调控Fas/FasL系统表达在抑制乳腺癌细胞增殖中的作用。方法:人乳腺癌细胞MCF-7(ER阳性)和MDA-MB-435(ER阴性)以VES联合他莫昔芬作用24和48 h,VES浓度为10和20μg/mL,他莫昔芬的浓度分别为1、5和10μmol/L。以MTT法测定对细胞增殖的抑制作用,以流式细胞仪分析细胞周期和细胞表面Fas/FasL表达的变化。结果:VES联合他莫昔芬对乳腺癌细胞具有显著的抑制作用。药物联合作用后,乳腺癌细胞表现为明显的G0/G1期阻滞,MCF-7和MDA-MB-435细胞的凋亡率分别由(3.30±0.2)%和(4.88±0.9)%升高至(25.61±2.7)%和(23.33±1.1)%,流式细胞仪提示,MCF-7和MDA-MB-435细胞表面的Fas分别由50.07和46.29升高至116.72和136.68;FasL分别由51.85和57.43升高至146.82和127.90。结论:VES联合他莫昔芬对乳腺癌细胞具有显著的抑制作用,其机制可能与细胞表面Fas和FasL表达上调有关。
OBJECTIVE：To investigate the inhibitory effect of vitamin E succinate combined with tamoxifen on the proliferation of human breast cancer cells. METHODS： Human breast cancer cells were treated with VES combined with tamoxifen for 24 h and 48 h. The concentrations of VES were 10 μg/mL and 20 μg/mL and those of tamoxifen were 1 μmol/L. 5 μmol/L and 10 μmol/L. The inhibitory effect was measured with MTT. Then the cell cycle and cell surface Fas FasL expression were analyzed with flow cytometry. RESULTS： The co-administration of VES and tamoxifen had significant inhibitory effect on the growth of MCF-7 （ER positive） and MDA-MB-435 （ER negative） human breast cancer cells. The percentage of G0 G1 phase cells increased and the apoptotic rate rose from 3.30%±0.2% to 25.61%±2.7% and from 4.8% ± 0.9% to 23.33%± 1. 1% respectively after the co-administration of VES and tamoxifen. Cell surface Fas expression in cancer cells increased from 50.07 and 46.29 to 116.72 and 136.68 while FasL from ,51.85 and 57. 43 to 146.82 and 127.90 after treated with VES combined with tamoxifen. CONCLUSlONS： VES combined with tamoxifen has significant inhibitory effect on the growth of human breast cancer cells. The mechanism maybe relates to Fas and FasL upregulation on the surface of cancer cells.
出处
《肿瘤防治杂志》
2005年第16期1232-1236,共5页
China Journal of Cancer Prevention and Treatment
基金
上海市科委科技发展基金资助(024119105)
