摘要
目的:建立丹参药材中丹参素含量测定的方法.方法:色谱条件:色谱柱为C18柱,流动相为甲醇-0.5%冰醋酸水溶液(15:85),检测波长为280 nm.结果:其回归方程为Y=714 525.615 4X-274 34.826 6,r=0.999 9,丹参素在0.343～2.058μg范围内线性关系良好;重现性RSD为0.76%(n=5);平均回收率为97.95%,RSD为0.38%(n=5).结论:本方法灵敏、简便、准确可靠,可作为丹参药材中丹参素的含量测定标准.
OBJECTIVE To determine danshensu in Salvia miltiorrhiza with HPLC. METHODS The analytical column was C18, The mobile phase was methanol-0. 5 % acetic acid（15：85） and detection wavelength was 280 nm. RESULTS The regression equation was Y= 714 525. 615 4X - 274 34. 826 6,r=0. 999 9,The content of danshensu showed good linearity in the range of 0. 343-2. 058μg;RSD = 0. 76, （n = 5）;The average recovery was 97. 95%,RSD = 0. 38%（n = 5）. CONCLUSION The method is found to be simple,sensitive and accurate,It can be used as a standard of determining the concentration of Danshensu in Salvia miltiorrhiza.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2005年第9期832-833,共2页
Chinese Journal of Hospital Pharmacy
