摘要
建立了高效液相色谱分离测定粘毛鼠尾草中一种新吉玛烷型倍半萜，3β，6β，8α-三乙酰氧基-4β，5α-环氧吉玛-1-烯的方法：采用Kromasil C18反相色谱柱，以甲醇-水（体积比75：25）为流动相，流速0．8mL／min，检测波长204nm，在10min内分离检测该倍半萜。结果 线性范围0．065～32．5μg，相关系数0．999，检出限0．01μg，平均回收率98％，RSD3．1％。测得粘毛鼠尾草全草石油醚提取物中该吉玛烷倍半萜的含量为20.9mg／g。
A simple, rapid and accurate high-performance liquid ehronlatographie technique coupled with photodiode array detection was developed for the determination of a new germacrane sesquiterpene, named 3β, 6β, 8α-triaeetoxy-4β, 5α -epoxygermaer- 1 （10） E-ene. HPLC was performed within 10 min on a Kromasil C is column （250 mm × 4.6 mm. i. d, 5μm） using methanol - water（75 ： 25 by volume） as the mobile phase at a flow rate of 0.8 mL/min, and 204 nm as the detection wavelength. The result showed that there was a good linear relationship in the range of 0. 065 - 32.5 μg with the correlation coefficient being 0. 999. The detection limit was 0.01 μg, and the mean recovery was 98% with a relative standard deviation of 3.6%. The content of germaerane sesquiterpene in the petroleum ether extract from the whole plant of Salvia robonvskii maxim was 20.9 mg/g.
出处
《分析测试学报》
CAS
CSCD
北大核心
2005年第5期98-100,共3页
Journal of Instrumental Analysis
基金
中国科学院百人计划项目基金
国家自然科学基金资助项目(NSFC20021001)
甘肃省自然科学基金资助项目(ZS021-A25-011-Z)
关键词
植物成分分析
高效液相色谱法
唇形科
粘毛鼠尾
吉玛烷倍半萜
Analysis of plant constituents
High performance liquid chromatogaphy
Labitatae： Salvia robor wskii maxim
Germacrane sesquiterpene
