摘要
对雪腐格氏霉（Ｇｅｒｌａｃｈｉａｎｉｖａｌｉｓ）产毒培养基的筛选结果表明，适宜菌株生长和产毒的液体培养基为马铃薯蔗糖培养液（ＰＳＢ）和添加１％蛋白胨的察氏（Ｃｚａｐｅｋ’ｓ）培养液。固体培养物玉米粒上的粗毒素产量远高于液体培养基。适宜茵株生长的条件是２０℃，ｐＨ６～７，黑暗和静止培养，适宜产毒的条件是１０℃，ｐＨ７～８，光暗交替和静止培养；培养滤液和从固体培养物中浸提的粗毒素对小麦种子均有毒性，浸提的粗毒素的毒性远高于培养滤液。粗毒素对小麦胚根生长的抑制作用与浓度密切相关，与生长率的相关系数为─０．８６７７（Ｐ＜０．０１）。
The result of the toxin-promoted media selection shows that liquid media fit for the growth and toxin-promotion of Gerlachia nivalis is PSB and Czapek's liquidmeditim(1%pepton extract),the output of crude toxin extracting from corn grain solidmedia is much higher than from liquid media.The condition for the grcwth of Gerlachia nivalis is a still culture in 20℃,pH 6～7,and darkness;the condition fit for its toxin-pro-motion is a still culture in 10℃,pH 7～8,light and darkness alternation,Liquid culture filtrate and crude toxin extraction from solid culture are all toxin to wheat seeds withthe higher toxicity of the former.It is revealed that the correlation exsits between the in-hibition of crude toxin to the growth of radicals and its concentration.And the correla-tion coefficient of the concentration to the growth rate is-0.8677(P<0.01).
出处
《西北农业大学学报》
CSCD
1996年第4期6-9,共4页
Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)
基金
国家"八五"攻关课题
关键词
雪腐格氏霉
毒素
培养条件
Gerlachia nivalis,toxin,culture condition
