摘要
以珍珠岩作基质固体培养六种不同基因型玉米自交系材料,设置0.25mol/L KH2PO4全磷(对照)、10μmol/L KH2PO4(低磷)、1μmol/L KH2PO4(极低磷)三种处理,于四叶一心时测定叶绿素含量、SOD活性、POD活性以及MDA含量的变化.各自交系在低磷和极低磷胁迫时,叶绿素含量都升高.缺磷时不同自交系的根和叶的SOD及POD的变化趋势不一致:自交系04099和04417叶片和根系的SOD和POD活性与对照相比均增加;04065叶片和根系的SOD活性比对照升高,但是POD活性比对照降低;04419在两个缺磷水平处理下叶片和根系的SOD活性较对照下降,叶片的POD活性较对照上升,但根系的下降;04242经两个缺磷水平处理后叶片和根系的SOD活性下降,叶片POD活性在低磷处理下上升,极低磷时下降,根系POD活性降低;04088 SOD活性变化与04242相同,但是低磷和极低磷下的叶片POD活性比对照增加,根系POD活性在低磷条件下降低,极低磷下上升.玉米的膜脂过氧化程度在缺磷处理时发生改变,低磷和极低磷处理时所有自交系的叶片和根系MDA含量也发生不了同程度的变化.综合分析,初步判断自交系04099和04065是耐低磷胁迫能力较高的材料,有进一步研究的价值.
Under solid cultivating with perlite substrate, six maize inbred lines were subjected to 3 phosphorus deficiency levels： 1） Full P level （CK） with 0.25mol/L KH2PO4, 2） Low P level （LP） with 10μmol/L KH2PO4, 3） Extremely low P level （ELP） with lμmol/L KH2PO4. The chlorophyll content, MDA content, POD activity and SOD activity were investigated. Under LP and ELP treatments, chlorophyll content of all inbred lines increased with certain degrees, but activities of POD and SOD showed different trends of changes in different lines. SOD and POD activities in leaves and roots of lines 04099 and 04417 all increased in LP and ELP treatments. Trend of SOD activity changes in 04065 was the same as those of lines 04099 and 04417, but its POD activity change trend was contrary to those of 04099 and 04417. Under two low -phosphorus levels, SOD activities in leaves and roots of 04419 decreased. Its leaf POD activity increased, while root POD activity declined under P deficiency conditions. P deficiency decreased both leaf and root SOD activities of line 04242, while leaf POD activity increased under LP condition and decreased under ELP condition, but root POD activity decreased under both LP and ELP conditions. Trend of SOD activity changes of line 04088 was the same as line 04242. P deficiency enhanced leaf POD activity of line 04088, but the root POD decreased under LP condition and increased under ELP condition. Phosphorus deficiency also changed the membrane lipid peroxidation conditions. Both leaf and root MDA contents showed certain extents of changes under LP and ELP treatments. In summary, lines 04099 and 04417 can be preliminarily regarded as low-phosphorus-tolerant maize inbred lines.
出处
《西华师范大学学报（自然科学版）》
2005年第3期257-261,共5页
Journal of China West Normal University(Natural Sciences)
基金
重庆市教委科学技术研究项目(020209)
关键词
玉米
磷胁迫
生理生化
maize
Phosphorus deficiency
biochemical and physiological characteristics
