摘要
目的研究缺氧条件下肝癌细胞系HepG2表达血管内皮生长因子(VEGF)的变化。方法分别在缺氧(缺氧组)和有氧(对照组)条件下培养HepG2细胞,采用免疫组织化学染色、MTT比色、细胞计数和ELISA方法,检测2组VEGF表达、HepG2细胞生长情况和培养上清液中VEGF含量。结果缺氧培养可抑制HepG2细胞的生长,缺氧培养48和72h后,HepG2细胞的生长速度明显低于对照组相应时相(P<0.05,P<0.01);缺氧培养24和48h后,HepG2细胞上清液中VEGF含量明显高于对照组相应时相(P<0.05,P<0.01)。结论缺氧可以促进肝癌细胞HepG2表达分泌VEGF,这可能是经导管肝动脉化疗栓塞术后VEGF高表达的原因。
Objective To investigate the change of vascular endothelial growth factor （VEGF） expression in HepG2 cells under hypoxia. Methods HepG2 cells were cultured under hypoxia（hypoxia group） and normal condition （control group）. VEGF expression of HepG2 cells was examined by immunohistochemical staining, The growth of HepG2 cells was examined by MTT colorimetry and cell count. VEGF level in the culture medium was measured by ELISA. Results After 48 h and 72 hof culture, the growth rate of HepG2 cells in hypoxiagroup was lower than that in control group （P〈0.05）. The cell count in hypoxia group （2.51×10^4/μl and 2. 69 ×10^4 /μl. respectively） was much lower than that in control group（3.01×10^4/μl and 3.52 ×10^4/μl） after 48h and 72h of culture （P〈0.05）. In hypoxia group. VEGF level in the culture medium after 24 hand 48 h was higher than that in control group （P〈0.05. P〈0.01）. Conclusion Hypoxia may enhance the VEGF expression in HepG2 cells and this could be the reason of high expression of VEGF after transcatheterized hepatic arterial chemoembolization.
出处
《中国普外基础与临床杂志》
CAS
2005年第5期477-479,共3页
Chinese Journal of Bases and Clinics In General Surgery
关键词
肝细胞癌
血管内皮生长因子
经导管肝动脉化疗栓塞术
Hepamcellular carcinoma Vascular endothelial growth factor Transcatheterlzed hepaticarterial chemoembolization
