摘要
目的探讨表皮生长因子(EGF)对急性胰腺炎(AP)大鼠胰腺保护作用的机理。方法SD大鼠72只,随机均分为3组:对照组、AP组及AP-EGF组。AP-EGF组动物在诱导致AP模型后皮下注射EGF0.1μg/g体重,其余2组动物皮下注射相同体积的生理盐水。各组动物分别于术后6、12及24h剖杀取材(每个时相8只动物),经心脏取血4ml,其中2ml采用全自动生化分析仪检测血清淀粉酶,其余用于检测血浆中丙二醛(MDA)含量;以干纱布吸沾腹水,腹水量为浸满腹水的纱布重量与干纱布重量之差;各时相剖腹作大体观察,切取相同部位的胰腺组织5g检测其MDA含量,并观测胰腺组织病理学改变和细胞凋亡。结果AP-EGF组动物胰腺病理损伤较AP组轻;术后12及24h,AP-EGF组动物腹水量显著低于AP组〔(4.53±1.29)g vs(6.58±1.47)g、(7.64±1.85)g vs(11.96±2.13)g,P<0.05,P<0.01〕,AP-EGF组动物血清淀粉酶也显著低于AP组〔(142.0±8.3)U/L vs(187.9±10.4)U/L、(194.3±10.4)U/L vs(253.3±8.6)U/L,P<0.05,P<0.01〕;术后24h,AP-EGF组动物血浆中MDA含量为(2.34±0.23)μmol/L,胰腺组织中MDA含量为(5.21±1.46)μmol/g,均显著低于AP组的(3.15±0.38)μmol/L和(7.68±1.63)μmol/L,P<0.05,P<0.01;术后各时相,AP-EGF组动物胰腺细胞凋亡指数显著高于AP组〔(16.22±3.53)%vs(7.35±1.04)%、(11.67±2.40)%vs(4.81±0.86)%、(6.38±1.42)%vs(1.97±0.21)%,P<0.01〕。结论EGF能减轻AP时大鼠胰腺的病理损伤,迅速恢复血清淀粉酶正常活性,降低AP大鼠血浆及胰腺组织中MDA含量,通过诱导AP时腺体细胞的凋亡发挥对胰腺的保护作用。
Objective To investigate the protective effects of epidermal growth factor （EGF） on pancreas of rats with acute pancreatitis（AP）. Methods Seventy-two male Sprague-Dawley rats were randomly divided into 3 groups： Control group, AP group and AP-EGF group. Subcutaneously injection of EGF （0.1 μg/g） were given to animals in the AP-EGF group after the establishment of the model of AP. The other two groups of animals received the same volume of saline. At 6 h, 12 h and 24 h after induction of AP, 8 animals in each group were sacrificed respectively, 4 ml of blood sample was withdrawn from heart,2 ml for the analysis of amylase activity and 2 ml for MDA content in serum. Ascites was sucked with dry gauzes and was weighed thereafter. Changes of pancreas morphology were evaluated at every time point. The same part of pancreas was removed for measurement of MDA content, apoptotic index （AI） and histologic changes. Results Histologic injury of the animals in the AP-EGF group was milder than that in the AP group. Ascites weight in the AP-EGF group decreased significantly compared with that in the AP group at 12 h and 24 h [（4.53± 1.29） g vs （6.58±1.47） g, （7.64± 1.85） g vs （11.96±2.13） g, P〈0.05,P〈0. 013. Amylase activity in the AP-EGF group also decreased significantly compared with that in the AP group at 12 h and 24 h [（142.0±8. 3） U/L vs （187.9±10.4） U/L, （194.3±10.4） U/L vs （253.3±8. 6） U/L, P〈0.05,P〈0.013. MDA content in plasm [（2. 34±0. 23）μmol/L vs （3. 15±0. 38）μmol/L, P〈0.053 and in pancreas[（5. 21±1. 46） μmol/g vs （7.68±1.63）μmol/g, P〈0. 013 in the AP-EGF group decreased significantly compared with those in the AP group at 24 h. AI of pancreas in the AP-EGF group increased significantly compared with that in the AP group after operation [（16.22±3.53）% vs（7.35± 1.04） % ,（11.67±2.40）% vs（4.81±0.86）%, （6.38±.42）% vs （1.97±0.21）%, P〈0. 01]. Conclusion EGF may accelerate the restoration of pathologic injury and alleviate the hemorrhage and edema of pancreas. It may also depress MDA content in plasm and in pancreas so that to lessen oxidative damage. EGF may protect pancreas by inducing cellular apoptosis.
出处
《中国普外基础与临床杂志》
CAS
2005年第5期433-437,共5页
Chinese Journal of Bases and Clinics In General Surgery
关键词
表皮生长因子
急性胰腺炎
腹水
丙二醛
凋亡
Epidermal growth factor Acute pancreatitis Ascites Malondialdehyde Apoptosis
