摘要
目的研究白细胞介素18(IL-18)在小鼠葡聚糖硫酸钠(DSS)结肠炎结肠粘膜中的表达及作用。方法免疫组化染色检测IL-18在小鼠DSS结肠炎中的表达。ELISA检测DSS结肠炎结肠上皮细胞(IEC)裂解液或培养上清液对脾单个核细胞(SMC)IL-2、IFN-γ分泌的作用。WesternBlot检测IEC裂解液或培养上清液中IL-18的表达。结果DSS结肠炎IEC及部分固有膜单个核细胞表达IL-18。刺激实验及WesternBlot显示IEC裂解液或培养上清液中均含IL-18阳性蛋白,IL-18诱导IFN-γ的产生。结论DSS结肠炎IEC表达和分泌前体IL-18及活性IL-18,可能在DSS结肠炎的发病中起重要作用。
Objective Interleukin-18 （IL-18） is a proinflammatory cytokine that induces Th1 cytokines production and may play a role in the pathogenesis of inflammatory bowel diseases. This study was aimed at detecting the expression and role of IL-18 in colonic mucosa of dextran sulfate sodium （DSS） colitic mice. Methods Expression of IL-18 in colon of DSS colitis was determined by immunohistochemistry. Extract and supernatant of intestinal epithelial cells （IEC） from colon of DSS colitis mice were used to stimulate splenic mononuclear cells （SMC） ; IFN-γ and IL-2 concentrations in supernatant of SMC were measured by ELISA. The levels of expression of IL-18 in extract or supernatant of IEC were examined by Western blot. Results Colon epithelial cells and part of lamina propria mononuclear cells were stained positive for IL-18 antibody. Stimulation assay and Western blot indicated that IL-18 positive proteins were present in both IEC extract and supernatant. Conclusion Colon epithelial cells in mice with DSS colitis express and secret pro IL-18 and active IL-18.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2005年第5期676-678,共3页
Journal of Sichuan University(Medical Sciences)
基金
国家自然科学基金(批准号30270606)资助
