摘要
以黄花水仙和南日岛水仙为材料,对2个水仙品种的组培快繁技术进行了研究.结果表明:以低温处理1个月的带鳞茎盘基部的鳞片为外植体,接种于MS+0.5 mg.L-1BA+0.1 mg.L-12,4-D的诱导培养基和MS+1.0 mg.L-1BA+0.5或1.0 mg.L-12,4-D的继代增殖培养基中,诱导黄花水仙形成的小鳞茎数量多,增殖率高;南日岛水仙以MS+0.5 mg.L-1BA+2.0 mg.L-1NAA的诱导培养基和MS+1.0 mg.L-1BA+0.5或1.0 mg.L-12,4-D的增殖培养基效果较好.生根培养基为1/2MS+0.05 mg.L-1NAA,移栽后成活率很高.
The study was carried out to develop a technological system for rapid propagation of Huanghua and Nanridao narcissus in vitro. The result indicated that segments of the bulbs with basal scales and a portion of the plate, which were pretreated under 4℃ for a month, were well used as explants. MS medium containing 0.5 mg·L^-1 BA and 0.1 mg·L^-12,4-D or 0.5 mg·L^-1 BA and 2.0 mg·L^-1 NAA was suitable for induction of bulblets of Huanghua or Nanridao narcissus, respectively. The MS medium, supplemented with 1.0 mg·L^-1 BA and 0.5 mg·L^-1 or 1.0 mg·L^-12,4-D, could be used for subculture of Huanghua and Nanridao narcissus. A high rate of viability was obtained in a rooting induction medium of 1/2 MS added with 0.05 mg·L^-1NAA for both narcissus.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2005年第3期313-317,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省推广基金项目(0202B9)
关键词
中国水仙
组织培养
快速繁殖
Narcissus tazetta Linn. var. chinensis Roem
in vitro culture
rapid propagation
