摘要
RAPD (random amplified polymorphic DNA) markers were generated from filaments of 15 Porphyra lines representing four important groups, P. yezoensis, P. haitanensis, P. katadai var. hemiphylla and P. oligospermatangia . Among the total 69 fragments generated by 6 selected primers (among 50 primers), 67 appeared to be polymorphic (97.1%). Cluster analysis based on the RAPD results was performed. The 15 Porphyra lines were divided into 3 groups. This result was consistent with that from taxonomy analysis. A DNA fingerprinting based on 8 bands amplified with OPN_02 and OPJ_18 was constructed and might be used in Porphyra variety identification. Five specific RAPD fragments of 5 Porphyra lines were isolated and cloned into pGEM_T easy vector. These five RAPD fragments may be useful in germplasm identification and property protection of Porphyra .
用RAPD技术对 4类紫菜 (Porphyrayezoensis,P .haitanensis,P .katadaivar.hemiphylla和P .oligosper matangia)的 15个无性系丝状体进行了遗传多样性分析 ,从 5 0个OPERON引物中经过初筛 ,其中 6个引物可以扩增出稳定的可重复的图谱。这 6个引物共扩增出了 6 9条带 ,多态性比例达 97.1%。根据RAPD结果将这 15个无性系聚类为 3个群 ,此聚类结果与分类地位基本相符。利用来自引物OPJ_18和OPN_0 2扩增的 8条多态性条带构建了紫菜的DNA指纹图谱 ,该图谱可以方便地应用于紫菜无性系的种质鉴定。本研究还回收并克隆了 5个紫菜无性系的 5条特异片段 ,将用于无性系鉴定、种质资源保护和产权保护。
基金
"8 63"OceanologyProjectofChinaHigh_TechProgram ( 819_0 3_0 2 )
