摘要
采用紫外光谱法和电化学法研究了水杨酸(SA)与鲱鱼精DNA的相互作用。紫外吸收光谱法结果表明,SA与DNA的相互作用为嵌插方式。在pH 5.8的NaH2PO4-Na2HPO4缓冲液中对SA进行循环伏安扫描,SA在玻碳电极上于1.070 V(Ag/AgCl参比电极)有1个阳极氧化峰,随着DNA的加入,氧化峰电流降低,峰电位正移,进一步表明SA嵌插到DNA分子的双螺旋结构中,二者相互作用生成了一种非电活性的超分子复合物。通过电化学方法可计算出SA与DNA复合物的结合比为3∶1,结合常数β为2.53×1012。
The interaction between salicylic acid（SA）and DNA was investigated by electrochemistry and ultraviolet spectroscopy.The ultraviolet spectroscopy results showed that SA and DNA interacted in an intercalative way.Then SA was further studied by cyclic voltammetry in NaH2PO4-Na2HPO4 buffer solution,pH5.8.SA had a DPV oxidation peak at the potential of 1.070 V（vs.Ag/AgCl）on the glassy carbon electrode.The presence of DNA led to the decrease in the oxidation peak current of SA and the positive shift in the potential.The results indicated that SA was intercalated into the helix structure of DNA and forms an electrochemically inactive supermolecular complex DNA：mSA.Electrochemical method showed a binding ratio of 3∶1 between SA and DNA in the complex,and the binding constant wasβ=2.53×1012.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第2期225-228,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家自然科学基金项目(31070403)
关键词
水杨酸
DNA
电化学
紫外光谱
salicylic acid
DNA
electrochemical
ultraviolet spectroscopy
