摘要
目的:观察局灶性脑缺血再灌注(FCIR)大鼠大脑皮质Caspase-3的表达.方法:雄性SD大鼠42只,分为假手术组和缺血再灌注(IR)组,IR组又分为缺血2 h再灌注2 h、6 h、12 h、24 h、48 h、72 h组,每组6只.IR组采用线栓法建立FCIR模型,假手术组仅插线1 cm;于脑视交叉平面向后7～11mm处取脑组织.HE染色观察各个时间点细胞形态学变化,免疫组织化学SP法观察不同时间Caspase-3的表达,TUNEL法检测细胞凋亡.结果:脑缺血2 h再灌注6 h Caspase-3表达升高,再灌注24 h达到高峰,再灌注72 h开始下降.凋亡细胞数的变化与Caspase-3表达变化一致.结论:Caspase-3参与了脑缺血再灌注神经元的死亡过程,该作用可能与细胞凋亡有关.
Aim ： To observe the expression of Caspase-3 after focal cerebral ischemia and reperfusion in rats brain cortex. Methods：The focal cerebral ischemia and reperfusion model was prepared by thread embolism of middle cerebral artery （ MCA）. The neuron morphologic change was studied by HE staining. The expression of Caspase-3 in ischemia cortex was determined using immunohistochemical technique. Cell apoptosis was detected using TUNEL. Results： In the process of reperfusion after 2 h ischemia, the expression of Caspase-3 began to increase at 6th h, reached peak at 24th h, and decreased at 72nd h. The change of apoptotic cells was accordant with that of Caspase-3. CoNclusioN ： Caspase-3 regulates the cell apoptosis,which plays a vital role in the injury of rat ischemia and reperfusion.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2005年第5期838-839,共2页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省自然科学基金资助项目511044000
