摘要
【目的】探讨一种改良IME-FICC(免疫磁珠富集-免疫荧光细胞化学)法检测乳腺癌外周血微转移癌细胞及其意义。【方法】通过与磁珠共价结合的表皮细胞黏附分子(EpCAM)抗体富集外周血中表达EpCAM的癌细胞,结合免疫荧光化学法检测细胞角蛋白(CK)8/18阳性的癌细胞(呈绿荧光)。放置MCF-7乳腺癌细胞于正常人外周血标本中以检验本方法癌细胞回收率及敏感度,检测20例正常女性志愿者及12例Ⅳ期乳腺癌患者治疗前的外周血标本,探索其特异性及临床应用可行性。【结果】免疫磁珠富集后癌细胞回收率达86%～93%,可在107外周血单个核细胞中检测到1个癌细胞,特异性为100%。Ⅳ期乳腺癌患者外周血中CK8/18阳性细胞检出率75%。【结论】改良IME-FICC法是一种简便、省时、敏感性及特异性高的检测方法,有较大的临床应用前景。
[Objective] To establish the method of modified immunomagnetic enrichment combined with fluorescent immunocytochemistry to detect micrometastases in peripheral blood of breast cancer and study its significance. [Methods] The magnetic beads covalently binding epithelial cell adhesion molecule （EpiCAM） antibody （Ab） can enrich the tumor cells in peripheral blood expressing EpiCAM , combined with the method of fluorescent immunocytochemistry to detect the tumor cells expressing cytokeratin 8/18 （stained with green fluorescence） . The tumor cell recovery rate and the sensitivity of the detection method were evaluated by sparking the MCF-7 breast carcinoma cell into the blood samples of volunteers. The specificity and the possibility of clinical usage of the detection method were also determined by comparing the twenty peripheral blood samples of the normal volunteers and the twelve blood samples of the patients with advanced breast cancer. [ Results ] The tumor cell recovery rate of immunomagnetic ceU enrichment ranged from 86% to 93%. One tumor cell in 107 peripheral blood mononuelear cells can be detected, with a specificity of 100%. Circulating tumor cells can be detected in 75% of the patients with stage IV breast cancer. [Conclusion] The study shows the technique is a time-saving, easily- performed, highly sensitive and specific method. The technique shows potentially great chnical value and further clinical trial is warranted.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2005年第5期596-599,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省医学科学研究基金资助项目(B2005052)
关键词
乳腺癌
微转移
免疫磁珠富集
免疫细胞化学
breast cancer
micrometastases
immunomagnefic enrichment
immunoeytoehemistry
