摘要
96头36日龄杜长嘉(杜洛克×♀长嘉)断奶仔猪,按体质量相近原则随机分成4个处理组,每个组设3个重复,每个重复8头猪(公母各半)。对照组(处理1)饲喂不添加任何锌源的基础饲料(DE 13.25 M J/kg,CP 18.5%),处理2、处理3、处理4分别饲喂添加硫酸锌(Zn 100 m g/kg)、氧化锌(Zn 3 000 m g/kg)和纳米氧化锌(Zn 100 m g/kg)饲粮。根据已报道的猪抗菌肽PR-39和看家基因β-肌动蛋白(-βactin)基因序列,分别设计PR-39和-βactin的引物,探讨了PCR体系中适宜的M gC l2浓度、循环次数,以及2对引物间竞争情况。以此构建一优化的半定量RT-PCR法。以β-actin为内标,研究了不同锌源对仔猪抗菌肽PR-39基因表达的影响。结果显示,与对照组相比,高氧化锌组(Zn 3 000m g/kg)显著提高了抗菌肽PR-39基因的表达量,提高量为378.26%(P<0.01),硫酸锌组(Zn 100 m g/kg)和纳米氧化锌组(Zn 100 m g/kg)也分别使抗菌肽PR-39基因的表达提高了38.4%和23.91%,但统计结果差异不显著。
Ninety-six Duroc×Landrace ×TaiHu weaning piglets （♂ Duroc ×♀ Landrace × TaiHu ） of 36 days were divided into four treatment groups randomly by weight and each group included three repeats of eight piglets. The control（treatment 1） received the basal dietary（DE 13.25 MJ/kg,CP 18.5%） that did not contain any zinc. Treatment 2,treatment 3 and treatment 4 received dietary contained zinc sulfate（100 mg/kg Zn）,zinc oxide（3 000 mg/kg Zn） and nanometer zinc oxide（100 mg/ kg Zn） respectively. The primers for PR-39 and β-actin were designed respectively according to the reported porcine antibacterial peptide PR-39 gene and housekeeping gene β-actin sequences,the suitable MgCl2 concentration,cycles,and the competition between the two pairs of primers in PCR system were discussed. So an optimized semi-quantitative RT-PCR was constructed ,β-actin used as inner control,to detect effect of different source of zinc on antibacterial peptide PR-39 gene expression in piglet. The results indicated that ,compared with control group,zinc oxide（3 000 mg/kg Zn） increased the gene expression of antibacterial peptide PR-39 markedly by 378.26% （P〈0.01）;Zinc sulfate（100 mg/kg Zn） and nanometer zinc oxide（100 mg/kg Zn） also increased the gene expression of antibacterial peptide PR-39 by 38.40% and 23.91% respectively,but statistic result was not significant.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2005年第5期523-526,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30040036)
浙江省人才专项基金项目(RC01053)
