摘要
核糖核酸酶(RNases)可以将衰老的植物组织中的核糖核酸降解释放出磷元素,使它能够运送到幼嫩部位被重新利用。许多核糖核酸酶基因的表达受磷饥饿的正调控。利用已有的EST序列,从普通小麦“小偃54”中分离了3个核糖核酸酶基因的cDNA序列。这3个基因预测的氨基酸序列与S-核糖核酸酶和S-like核糖核酸酶(类核糖核酸酶)的氨基酸序列有较高的同源性。WRN1的表达受磷饥饿和衰老的负调控,而WRN2和WRN3受磷饥饿的正调控。
In plants, RNase can degrade RNA to release Pi in senescent organs, which can be reused by young organs. The expression of many S-like RNase genes were up-regulated by phosphate starvation. Using the ESTs, three S-like RNase gene cDNAs were isolated from common wheat by RT-PCR. The predicated amino acid sequences of the three sequences were found having high similarities with those S-RNases and S-like RNases in other plant species. The expression of WRN1 was down-regulated by phosphate starvation and leaf senescence, while WRN2 and WRN3 were up-regulated by phosphate starvation.
出处
《作物学报》
CAS
CSCD
北大核心
2005年第9期1115-1119,共5页
Acta Agronomica Sinica
基金
科技部973项目(2004CB117200)
863项目(2003AA207080)资助。
关键词
磷饥饿
普通小麦
类核糖核酸酶
Phosphate starvation
Common wheat （ Triticum aestivum L. ）
S-like RNase
