摘要
在克隆获得磷高效相关转录因子基因GmPTF1的基础上,构建基因的超表达载体pGN-GmPTF1和无标记(Marker-free)表达载体pX6-GmPTF1;利用农杆菌介导子叶节与花粉管通道转化技术,将表达载体pGN-GmPTF1和pX6-GmPTF1分别转入冀豆12、冀豆16和五星1号大豆品种。转化后的大豆植株经PCR检测,有11株转化植株可扩增出目的条带,其中5株转有pGN-GmPTF1载体和6株转有pX6-GmPTF1载体,表明GmPTF1转录因子基因已初步整合至大豆基因组中。
The over-expression vector pGN-GmPTF1 and marker-free expression vector pX6-GmPTF1 were constructed in this study base on the cloning and identification of GmPTF1,a soybean high phosphorus-efficiency transcription factor gene,and the vectors were introduced into different soybean varieties（Ji-dou 12,Ji-dou 16,Wu-xing 1）by Agrobacterium-mediated cotyledonary-node and pollen tube pathway transformation methods.PCR analysis results of transformation plants showed that the GmPTF1 was successfully incorporated into the soybean genome.Total five PCR positive plants of vector pGN-GmPTF1 and six PCR positive plants of vector pX6-GmPTF1 were obtained from T0 generation,respectively.
出处
《华北农学报》
CSCD
北大核心
2011年第6期27-31,共5页
Acta Agriculturae Boreali-Sinica
基金
国家转基因重大专项资助项目(2009ZX08004-004B)
国家自然科学基金项目(31071441)
河北省自然科学基金项目(C2010000749)
河北省教育厅科学研究项目(2009)
关键词
大豆
转录因子
载体构建
遗传转化
Soybean
Transcription factor
Vector construction
Genetic transformation
