摘要
从对Ｖｅｒｔｉｃｉｌｌｉｕｍａｌｂｏ－ａｔｒｕｍ感抗性不同的两株近等位基因番茄获得细胞悬浮培养物Ｓ－ＧＣＲ２６和Ｒ－ＧＣＲ２１８。用．ａｌｂｏ－ａｔｒｕｍ激发子处理后，敏感性的Ｓ－ＧＣＲ２６未表现出明显的反应性氧改变。抗性的Ｒ－ＧＣＲ２１８则响应以反应性氧迸发。反应性氧迸发中的细胞，其反应性氧累积量与激发子浓度的关系呈近Ｓ形。悬浮介质、某些无机离子、培养物的生长状况和培养日龄等因素均影响细胞对激发子的反应性氧迸发响应。
The oxidative burst in two near isogenic tomato cell cultures induced by the elicitor from V. albo-atrum was investigated. The oxidative bursts of resistant and susceptible cell lines in Gamborg's B5/ 2% sucrose or in 2% sucrose only were compared (Figs. 1, 2 ).Resistant cells showed a much higherlevel of reactive oxygen 20 min afterthe elicitor was added in two media.Cell age(Fig. 6) growth density, shaking (Fig. 5), concentration of the elicitor (Fig. 4 ), some oxidase (Fig. 8) andcathions (Fig. 3 ) etc. affect the Peakvalue of oxidative burst in resistanttomato cell cultures. However, the pattern of the time course of oxidativeburst was the same. The reactive oxygen species were increased rapidly atabout 10 min and up to its Peak at 20min. There was always a difference inoxidative burst between the two nearisogenic tomato cell cultures and the relative light units in resistant cell cultureexhibited by luminol chemiluminscencewas 100 to 1000 times that in the susceptible one (Figs. 1, 2 ). The dependence of oxidative burst on concentration can be expressed by a sigmoidcurve(Fig. 4 ). When the concentrationof the elicitor was less than 1 μg/ml,the level of oxidative burst was verylow. It increased rapidly with the concentration the elicitor and up to a maximium value of relative light units between 25 to 30 μg of elicitor per ml ofcell suspension at 25 min.
