摘要
以葡萄品种(Vitis vinifera)霞多丽(Chardonny)为材料,研究了外植体类型、不同培养基、不同激素及其质量浓度组合、暗处理时间对霞多丽丛生不定芽诱导的影响。结果表明,在所试的3类外植体中,仅从茎段诱导出不定芽。在所试的MS、NN1969及B53种培养基上,平均不定芽数差异不明显。当BA和IBA不同质量浓度组合时,霞多丽茎段均能诱导出不定芽,而且不同处理间不定芽诱导率差异显著,但每个外植体上诱导的平均不定芽数差异不明显,为0～2.5个。BA和IBA组合诱导的不定芽以单个芽为主。BA和NAA不同质量浓度组合对茎段不定芽诱导率和丛生不定芽数均有显著影响,当BA质量浓度为3.0mg·L-1、NAA为0.1mg·L-1时,不定芽诱导率和平均不定芽数达到最高,分别为9.7%和10.1个。由BA和NAA组合诱导的不定芽均为丛生不定芽,单个茎段诱导丛生不定芽数最高可达27个。将不定芽置于1/2MS+IAA0.2mg·L-1培养基上诱导生根,获得了完整的再生植株。
In order to induce multiple shoots from Chardonny grape （Vitis vinifera）explants, the effects of explants, media, hormone concentrations and dark treatment on the induction of multiple shoots were studied. The shoots were induced only from the stem explants, the induction frequency reached to 17.5%. There was no difference in the average number of shoots among 3 media of MS, NN 1969 and B5. The shoots were induced from the stems on the MS medium supplemented with BA and IBA, and there was significant difference in the frequency of shoot induction among different concentration treatments of BA and IBA. However, there was no significant difference in the average number of shoots and most of shoots induced were single shoot, indicated that there was no significant effect of the combination of BA with IBA on the induction of multiple shoots in this study. The significant effect of the combination of BA with NAA on both the induction frequency of shoots and the number of multiple shoots was observed. The highest induction frequency （9.7%）of shoots and the highest average number of shoots （10.1）were induced from the stems on the MS medium supplemented with 3.0mg·L^-1BA and 0.1 mg·L^-1 NAA, the highest number from one stem reached up to 27. The roots were induced from the shoots on the 1/2MS medium supplemented with 0.05 mg·L^-1 IAA.
出处
《果树学报》
CAS
CSCD
北大核心
2009年第2期222-225,F0002,共5页
Journal of Fruit Science
基金
农业部948项目(2006-G26)
