摘要
目的:研究增殖细胞核抗原(PCNA)的表达与人乳腺癌细胞凋亡的关系。方法:以乳腺癌细胞株MCF7/S(化疗敏感细胞株)为研究对象,应用MTT比色法检测阿霉素(ADR)对体外培养的MCF7/S细胞增殖抑制作用,末端标记(TUNEL)法检测ADR诱导乳腺癌细胞凋亡,免疫细胞化学法检测ADR作用前后PCNA的表达。结果:ADR抑制MCF7/S细胞增殖,呈剂量依赖性,IC50为0.128mg/L;ADR作用组MCF7/S细胞的凋亡率(Apoptoticrate,AR)为0.261,与对照组细胞的凋亡率0.0449相比明显增高(P<0.01);PCNA阳性表达率为0.3371,与对照组PCNA阳性表达率0.5152相比明显降低(P<0.01)。结论:乳腺癌肿瘤细胞的凋亡率(AR)和PCNA的阳性表达呈负相关;ADR能诱导MCF7/S细胞凋亡,并能抑制其增殖。
Objective：To investigate the relationship between proliferating cell nuclear antigen（PCNA） and cell apoptosis in human breast cancer. Methods：MTT colorimetric assay was applied to examine the growth inhibition, and apoptosis rates were determined by terminal-deoxynucleotidyl transferase mediated d-UTP nick end labeling（Tunel） ;The expressive levels of dephosphorylated RB protein and PCNA were detected with immunocytochemistry. Results：MTT assay that ADR inhibited proliferation of MCF-7/S cells in a dose dependent manner,the 50% inhibition concentration （ IC50 ） was 0. 128mg/L. Tumor cell apoptotic rate（AR） in ADR group （x =0. 261 ） was significantly higher than that in control group （x =0. 0449,P 〈0. 01 ） ,PCNA positive expression rate in ADR group （ x = 0. 3371 ） was significantly lower than that in control group （ x = 0.5152, P 〈 0.01 ）. Conclusion： There was significant negative association between AR and PCAN in ADR group and control group;ADR could include MCF-7/S cells apoptosis and inhibit MCF-7/S cells proliferation.
出处
《临床肿瘤学杂志》
CAS
2005年第4期374-376,共3页
Chinese Clinical Oncology
