摘要
目的:研究RB蛋白表达状况对乳腺癌细胞凋亡及其周期变化的影响。方法:以乳腺癌细胞株MCF-7/S为研究对象,采用免疫细胞化学法检测多柔比星(ADR)作用前后RB蛋白的表达状态;应用流式细胞术进行细胞凋亡和细胞周期分析。结果:加ADR前MCF-7/S细胞的细胞核磷酸化RB蛋白为阳性,去磷酸化RB蛋白为阴性。经不同浓度ADR处理后,随着ADR浓度的增加,MCF-7/S细胞去磷酸化RB蛋白表达量逐渐增高。流式细胞术分析结果表明,经0.25、2.0和5.0μg/m l ADR作用24 h后,MCF-7/S细胞凋亡率分别为17.1%、18.4%和25.9%。细胞周期分析表明,MCF-7/S细胞主要积聚于G1期。结论:去磷酸化RB蛋白表达与MCF-7/S细胞凋亡率呈正相关(r=0.998,P<0.05),同时MCF-7/S积聚于G1期。
Objective ：To study the effect of RB protein expression levels on apoptosis and cell cycle of breast cancer cell lines. Methods：Human breast cell lines （MCF-7/S） were evaluated. The expressive levels of dephosphorylated RB protein were detected with immunocytochemistry. Apoptosis rates and cell cycle were determined by flow cytometry （FCM）. Results ： Before treatment of ADR, phosphorylated RB protein was positive in MCF-7/S cell lines, but dephosphorylated RB protein was negative ; After treatment of different concentration ADR, when the concentration of ADR was increased, expression of dephosphorylated RB protein elevated accordingly in MCF-7/S. FCM assays indicated ： After 24 hours treatment of 0.25, 2, 5 p.g/ml ADR ,the rate of MCF-7/S was 17.1% , 18.4% , 25.9% respectively,the apoptotic rate of MCF-7/S was enhanced accordingly. Analysis of the cell cycle made it clear that after treatment of ADR, MCF-7/S ceils were accumulated in G1 phases. Conclusion： There were positive correlation between the expression levels of dephosphorylated RB protein and the apoptosis of MCF-7/S ceils, and MCF-7/S was accumulated in G1 phase of the cell cycle.
出处
《医学研究生学报》
CAS
2005年第9期773-775,i0012,共4页
Journal of Medical Postgraduates
基金
湖南省卫生厅科研基金资助项目(批准号:2001-Y58)
