摘要
确定了鸡肉中氯羟吡啶残留量的高效液相色谱分析方法.样品中的氯羟吡啶经乙腈提取,碱性氧化铝SPE柱纯化后,旋转蒸干,用流动相溶解残渣,过膜后进样;色谱柱采用Waters Symmetry C18柱(250 mm×4.6 mm,5 μm),磷酸盐缓冲液(pH 7.0)-乙腈(体积比90:10)为流动相,流速为1.0 ml/min,检测波长为270nm,柱温为25℃,进样量50 μl;鸡肉中氯羟吡啶的定量限为0.01 mg/kg;氯羟吡啶的测定在0.025～0.500μg/ml范围内具有良好的线性关系,平均回收率在80.6%以上,变异系数小于7.5%.
A new high-performance liquid chromatographic （HPLC） method for the determination of clopidol residue in chicken muscle was reported. The clopidol was extracted by acetonitrile. After the clopidol was purified by passing through Bond Elut B-Alumina solid-phase extraction cartridges, the eluant was evaporated （40℃） and residue was dissolved in mo- bile phase. A Waters Symmetry C18 colmun（250 mm× 4.6 mm,5μn）was employed for separation. The mobile phase consisted of phosphate buffer-acetonitrile （90： 10, v/v）, and the flow rate was 1.0 ml/min. The UV detection wavelength was set at 270 nm. The calibration graph was linear between 0. 025 μg/ml and 0.50μg/ml for clopidol. Recovery rates of clopidol was 80.6% - 96.3%, and RSD% was less than 7.5% （n = 5）. The method is simple, sensitive and rapid, and could be applied to the determination of clopidol in chicken muscle.
出处
《浙江农业学报》
CSCD
2005年第4期200-202,共3页
Acta Agriculturae Zhejiangensis
关键词
氯羟吡啶
残留
鸡肉
高效液相色谱法
clopidol
residue
chicken muscle
high-performance liquid chromatography（HPLC）
