摘要
对鸡肉组织中氯羟吡啶残留高效液相色谱方法进行了研究,建立了鸡肉组织中氯羟吡啶残留超高效液相色谱检测方法。取鸡肉样品,用乙腈提取,正己烷脱脂,过氧化铝B柱,用20ml甲醇洗脱,减压蒸干,残留物用甲醇溶解,紫外检测器在267nm波长下测定,流动相为乙腈:水(10:90,V/V)。氯羟吡啶浓度在10～1000μg/L范围内,呈良好的线性,相关系数>0.999,在10、50、100μg/kg三个添加水平,平均回收率60.5%～94.6%,日内变异系数在4.2%～10.2%之间,日间变异系数在6.6%～12.7%之间,方法检测限为5μg/kg,定量限为15μg/kg。
An ultra performance liquid chromatography （UPLC） method for determination of clopidol in chicken was developed. The clopidol residue in chicken was extracted by acetonitrile, defatted by n-hexane, purified by alumina B cartridges, analyzed by HPLC with UV detector at 267 nm and acetonitrile-water （10：90, V/V） solvent as mobile phase, and quantified by using external standard calibration curve. The results showed that there was a nice linear relationship （r 〉 0.999） when clopidol concentration was in the range of 10 to 1000μg/L. The average recoveries at three addition levels （10, 50 and 100μg/kg） were 60.5% to 94.6%. The within-day and inter-day variation coefficients were between 4.2% and 10.2% and between 6.6% and 12.7%, respectively. The limit of detection was 5μg/kg, and the limit of quantification was 15μg/kg.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第10期209-211,共3页
Food Science
关键词
氯羟吡啶
超高效液相色谱
鸡肉
残留
clopidol
ultra performance liquid chromatography （UPLC）
chicken
residue
