摘要
目的:观察环孢素(CsA)在体外对再生障碍性贫血(AA)的粒/巨噬系祖细胞(CFU-GM)和红系祖细胞(CFU-E)的作用,为临床应用提供实验依据。方法:采用RPMI1640琼脂和McCoy's5A甲基纤维素半固体平皿法,观察了CsA在体外对54例AA患者CFU-GM和CFU-E的影响,并以30例正常人作对照。结果:①AA患者的CFU-GM、CFU-E较对照组明显减低(P<0·01)。②CsA能明显促进AA患者CFU-GM、CFU-E的增殖,这种促增殖作用随CsA剂量增加而增加。③重型AA患者CFU-GM和CFU-E对CsA的有效率较慢性AA高。④CFU-GM和CFU-E值中、重度减低的AA患者对CsA的有效率较无CFU-GM和CFU-E生长的AA患者高。结论:CsA在体外可促进部分AA患者CFU-GM、CFU-E的增殖,故可作为治疗AA的一种有效方法。
Objective ： To observe the effect of cyclosporine A （CsA） in vitro on colony-forming unit-granulocyte macrophage （ CFU-GM ） and colony-forming unit-erythroid （CFU-E） in patients with aplastic anemia （AA） ,and to offer experimental evidence to potentialy clinical use of CsA. Methods：Bone marrow mononuclear cells from 54 AA patients were collected and cultured in RPMI 1640 agar and McCoy＇s 5A mythycellulose medium,respectively. The effects of CsA on CFU-GM and CFU-E from AA patients were compared with those from 30 normal controis. Results：①The numbers of CFU-GM and C FU-E in patients with AA were significantly decreased than those of normal controls （P 〈 0.01 ）. ②CsA could significantly promote the proliferation of CFU-GM and CFU-E in the patients ,and this proliferative effect was dependent on the dose of CsA. ③The response rate in severe AA patients to CsA was higher than that in chronic AA patients. ④The response rate of patients with severe and moderate decreases and with CFU-GM and CFU-E proliferation was higher than that of those patients without proliferation of CFU-GM and CFU-E. Conclusions：CsA could promote in vitro the proliferation of CFU-GM and CFU-E in some of the patients with AA,therefore,it could be regarded as an effective treatment of AA.
出处
《华北国防医药》
2005年第4期232-233,共2页
Medical Journal of Beijing Military Region
关键词
环孢素
贫血
再生障碍性
体外研究
干细胞
Cyclosporine
Anemia, aplastic anemia
In vitro study
Stem cell
