摘要
主要研究pH值、金属离子、温度对云芝漆酶活性和稳定性的影响,以及该酶底物浓度效应和Km值测定.实验结果表明:Cu2+、Co2+对漆酶有激活作用,而Ag+则有抑制作用;该酶最适pH为4.8,在pH4～4.8表现出较强的稳定性;最适反应温度为40 ℃,低于50 ℃时有较好的热稳定性;Km值为4.2×10-3 mol/L.
The effect of pH, metal ion and temperature on Coriolus versicolor laccase activity and stability was studied in the paper. Meanwhile, the substrate concentration effect of this laccase is also studied and Km is measured. The results showed： Cu^2＋ and Co^2＋ had activation but Ag^＋ had inhibition on activity of laccase. The optimum pH of the laccase was 4.8, and the laccase was stable from pH4 to 4.8. The optimum temperature of laccase was 40℃ ; and the laccase could work continuously under 50 ℃. The Km of the laccase measured 4.2× 10^-3 mol/L.
出处
《河南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第3期109-112,共4页
Journal of Henan Normal University(Natural Science Edition)
基金
河南省科技攻关项目(001200217)
河南省动物学重点学科资助项目
