期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

全基因组扩增技术的法医学应用价值及其结果评价 被引量：5

Practice Value of Whole Genome Amplification Technology to be Used in Forensic Science and Analysisof its Result
在线阅读 下载PDF
导出
摘要 微量模板DNA的分析,一直是法医学特别关注和急需解决的难题之一,近年发展起来的一系列技术为解决这一问题提供了新的可能途径,本文即对微量模板DNA分析技术的进展和目前逐步发展的全基因组扩增技术的法医学应用价值、结果评价进行浅议。 Genetic analysis from forensic microsamples is a urgent, difficult task in forensic science, because it is frequently limited by the amount of specimen available in forensic practice, much effort has been carried out to resolve this difficulty. Whole genome amplification （WGA） technology, which was developing quickly in these years, has been thought to be a powerful, reliable and efficient strategy in analysis of minute amount DNA on many fields. In this review, we discuss its application in forensic science.
作者 邓建强 侯一平
机构地区 司法部司法鉴定科学技术研究所 四川大学华西基础医学与法医学院
出处 《法医学杂志》 CAS CSCD 2005年第3期219-222,225,共5页 Journal of Forensic Medicine
关键词 法医学 DNA检验 微量分析 全基因组扩增 forensic medicine DNA analysis microassay whole genome amplification
分类号 D919 [医药卫生—法医学]
  • 相关文献

参考文献14

  • 1Kloosterman AD, Kersbergen P. Efficacy and limits of genotyping low copy number (LCN) DNA samples by multiplex PCR of STR loci[J]. J Soc Biol, 2003,197(4):351-359.
  • 2Morling N. Forensic genetics[J]. Ugeskr Laeger, 2003,165(9):922-925.
  • 3Hosono S, Faruqi AF, Dean FB, et al. Unbiased whole-genome amplification directly from clinical samples[J]. Genome Res, 2003 ,13(5):954-964.
  • 4Tanabe C, Aoyagi K, Sakiyama T, et al. Evaluation of a whole-genome amplification method based on adaptor-ligation PCR of randomly sheared genomic DNA[J].Genes Chromosomes Cancer,2003 ,38(2):168-176.
  • 5Hawkins TL, Detter JC,Richardson PM, et al. Whole genome amplification-applications and advances[J].Curr Opin Biotechnol, 2002,13(1): 65-67.
  • 6Kwok PY. Making random amplification predictable in whole genome analysis [J]. Trends Biotechnol,2002,20(10):411-412.
  • 7Satoh K, Takahashi K, Itoh Y, et al. Typing of DNA using the primer extension preamplification methodstudies of reliability of typing and detection limits[J].Nippon Hoigaku Zasshi, 1998,52(3):184-190.
  • 8Barbaux S, Poirier O, Cambien F. Use of DOP -PCR for the genotyping of low-concentration DNA samples[J]. Mol Med, 2001,79(5-6):329-332.
  • 9Dean FB,Hosono S,Fang L, et al. Comprehensive human genome amplification using multiple displacement amplification [J]. Proc Natl Acad Sci USA ,2002,99(8):5261-5266.
  • 10Leclair B, Sgueglia JB. STR DNA typing: increased sensitivity and efficient sample consumption using reduced PCR reaction volumes[J]. J Forensic Sci,2003 ,48(5):1001-1013.

二级参考文献17

  • 1CAll P, Whitaker J, Flaxman C, et al. An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA [J]. Forensic Sei Int, 2000, 112:17.
  • 2Gill P. Application of low copy number DNA profiling [ J ].Croatian Medical Journal, 2001, 42:229.
  • 3Cotton EA., AUsop RF, vip JL, et al. Validation of the AMPF1STR SGM PlusTM system for use in forensic casework[J]. Forensic Sci Int, 2000, 112:151.
  • 4Whitaker JP, Cotton EA, Gill P. A comparison of the characteristics of profiles produced with the AMPF1STR SGM Plus TM multiplex system for both standard and low copy number (LCN) STR DNA analysis [J]. Forensic Sci Int, 2001,123:215.
  • 5Budowle B,Smith JA, Moretti T, et al. DNA Typing Protocols: Molecular Biology and Forensic Analysis [ M]. Massachusetts: Eaton Publishing (USA) 2000.
  • 6Buder JM. Forensic DNA typing [ M]. London: Academic press (UK), 2001. 43.
  • 7Findlay I, Frazier R, Tayter A, et al. Single cell DNA fingerprinting for forensic applications [J]. Nature, 1997, 389:555.
  • 8Barbaro A, Faleone G, Barbaro A. DNA typing from hair shaft [J]. Progress in Forensic Genetics, 2000, 8:523.
  • 9Gill P, ivanov PL, Kimpton C, et al. Identifieation of the remains of the Romanov family by DNA analysis [ J ]. Nat Genet, 1994, 6 (1): 30.
  • 10Schmerer WM, Hummel S, Herrmann B. Optimized DNA extraction to improve reproducibility of short tandem repeat genotyping with highly degraded DNA as target [J]. Electrophoresis, 1999, 20 (1): 712.

共引文献28

同被引文献50

引证文献5

二级引证文献11

法医学杂志
2005年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部