摘要
本研究以内蒙古通辽地区蒙古牛为材料,构建了富集CA、CAG、GACC重复的牛微卫星文库,从中选取6条微卫星DNA序列设计引物,应用优化后的多重PCR反应条件分析32个个体的基因型,结果表明,具有特异性PCR扩增产物的8个位点中2个位点在所检测的群体中未表现出多态,另外6个位点在所检测的群体中等位基因数为2-5个.
In this study,Mongolian Cattle of Tongliao area constructed for the material enrichment of CA,CAG,GACC repeat of cattle microsatellite library.Their charaterazition and polymorphism were tested in a population of 32 Peking cattles.Two of the eight microsatellite loci were monomorphic,between two and five alleles were observed at the other polymorphic makers.
出处
《内蒙古民族大学学报（自然科学版）》
2011年第6期694-696,共3页
Journal of Inner Mongolia Minzu University：Natural Sciences
关键词
蒙古牛
微卫星
特性分析
Mongolian Cattle
Microsatellite loci
Characterization
