摘要
目的考察云南蜂胶精在泊洛沙姆温敏凝胶系统中的缓释效果.方法 (1)采用紫外分光光度法,以芦丁标准品做对照,测定云南蜂胶精提物中总黄酮含量;(2)通过正交试验设计,采用饱和水溶液法优选出蜂胶β-环糊精包合云南蜂胶的最佳工艺;(3)检测蜂胶包合率及包合物回收率2个指标,优选出最佳包合工艺;(4)以体外药物释放度为最终目标,考察该蜂胶环糊精包合物在泊洛沙姆温敏凝胶系统中的缓释效果.结果β-环糊精包合云南蜂胶的最佳工艺条件是:蜂胶:β-环糊精(1:2),包合温度70℃,包合时间1h.蜂胶包合率为43.75%,回收率为32%.药物在人工唾液内16 h释放度在99%以上,较未用β-环糊精包合的蜂胶的药物释放度有所延长.结论蜂胶β-环糊精包合后,从凝胶缓释体系中释放速度有所减缓,可持续释放16 h以上,达到缓释的目的.
Objective To optimize the technology of preparation propolis wrapped with cyclodextrin by using the orthogonal design method,and to study the in vitro drug release rate in the thermosensitive in situ Gel based on Poloxamer System.Methods(1)The amount of total flavone in Yunnan propolis extract was determined with ultraviolet spectrophotometry.(2)By using the orthogonal design method and saturated aqueous solution,we optimized the technology of preparation propolis wrapped with β-cyclodextrin.(3)We assayed the inclosure rate and the recovery rate of propolis,and optimized the technology.(4)We studied the sustained release effect of the propolis wrapped with cyclodextrin which in situ gel based on poloxamer system,using in vitro drug release as the ultimategoal.Results The optimum preparation conditions were determinted through orthogonal experiment:ratio of propolis to β-cyclodextrin was 1 to 2,bathing temperature was 70 ℃ and milling time was 1.0 h.Then the inclusion rate reached 43.75%,the recovery rate reached 32%.In artificial saliva,the target drug could sustained release more than 99% within 16 h,the drug sustained release effect is better than the non-β-cyclodextrin with propoils.Conclusion The release rate of propoils with β-cyclodextri from hydrogel becomes slow and controls delirery over 16 hours.
出处
《昆明医学院学报》
2011年第12期14-17,共4页
Journal of Kunming Medical College
基金
国家自然科学基金资助项目(30960424)
云南省自然科学基金资助项目(2007CA011)
关键词
云南蜂胶
Β-环糊精
牙周缓释
泊洛沙姆
Yunnan propoils
β-cyclodextrin
Periodontal release
Poloxamer
