摘要
复制了大鼠烧伤休克模型。用显微电视装置在放大4000倍条件下观察了自由基清除剂对大鼠脊斜肌微循环中白细胞附壁数及毛细血管开放数的影响。测定了组织含水量的变化及动物存活时间。结果表明,烧伤前或烧伤后立即给自由基清除剂可明显减少白细胞附壁粘着,毛细血管开放数增加,微循环明显改善,动物存活时间延长。SOD还有效的防止了烧伤后肺和皮肤含水量的增加,降低血管通透性.讨论了自由基在烧伤休克微循环紊乱发生中的作用。
The effect of free radicals scavengers (SOD and allopurinol) on the adherence of leuko- cytes and the blood flow of capillaries in spinotropizus muscle was observed in rats with burn shock. The changes in water content in heart, liver, lung, spleen, kidney and burned skin were measured. The survival time of the animals was recorded. The results showed that the number of the adherence of WBC decreased obviously, the amount of open capi- llaries increased, and the survival time of the animals wit thermal injury prolonged by pretreating with SOD and allopurinol. The results of SOD were better than that of allopu- rinol. SOD also prevented the increase of water content in the lung and the burned skin, while allopurinol did not have this effect. It was shown that the therapeutic effect of SOD would appear if the medicine were given before or immediately after burn, However when SOD was administered half an hour after burn, there was no effect on adherence of WBC. These resuts confirm that superoxide free radical may be responsible for the adherence of WBC and hypoperfusion of microcirculation during burn shock. The scavengers have an important role in preventing microcirculatory disturbances, but it should be used as early as possible after thermal injury in order to block the response chain caused by free radicals.
