摘要
报告了用反相ＨＰＬＣ测定临产妇静脉血及新生儿脐血中的地西泮浓度，采用甲喹酮为内标定量，地西泮浓度在５～５０００ｎｇ／ｍｌ范围内线性关性良好，最低检测浓度在ｓｎｇ／ｍｌ以下。绝对回收率在９８％以上，日内误差为９９．６４％±２．５％，日间误差为９８．３１％±３．１４％。测定结果表明，母血中地西泮浓度与新生儿脐血中浓度呈平行关系，脐血中地西泮浓度与母血中浓度之比为０．９１±０．２７。
With Rp-HPLC analysis method of diazepam in blood of parturient mother andnewborn’s umbilical cord,the content of diazepam in blood was determined with methagualone asinternal standard.The calibration curve was linear in the range from 5 ng/mI to 5000 ng/ml.Thelowest detectable concentration was less than 5 ng/m1.The absolute recovery was more than98%,the precision of diazepam from serum within-day was 99.64%±2.5%,and the precision ofdiazepam from serum day-to-day was 98.31%±3.14%,The resuIts showed that the concentration of diazepam in blood of parturient mother and newborn's umbilical cord was parallelized,therate of diazepam in the blood of mother and newborn's umbilical cord was 0. 9l±0. 27。
出处
《中国药学杂志》
CAS
CSCD
北大核心
1995年第7期421-424,共4页
Chinese Pharmaceutical Journal
