摘要
以肺吸虫成虫童虫冰冻切片抗原片做间接荧光抗体试验（ＩＦＡＴ）及免疫酶染色试验（ＩＥＳＴ）对比检测９４份肺吸虫感染者和４０例正常健康人血清，两法的阳性率分别为９３．６％、９５．７％和８９．１％、９３．６％，假阳性率均为０。ＩＦＡＴ和ＩＥＳＴ对比检测３４份血吸虫病人血清，３５份华支睾吸虫病人血清和３０份肺结核病人血清。ＩＦＡＴ出现的交叉反应率为１４．７％、５．７％、０和１４．７％、８．６％、０。ＩＥＳＴ出现的交叉反应率为５．９％、０、０和８．８％、５．７％、０。结果表明两法用于肺吸虫病的诊断均有较高的敏感性和特异性，而且抗原定位好，ＩＥＳＴ不需荧光显微镜或荧光设备。
Both indirect fluorescent antibody test(IFAT)and immunoenzymatic staining technique(IEST)with frozen sections of adult and adolescent Paragonimus westermani as antigen were employed in for detecting 94 cases with paragonimiasis and 40 healthy persons.The positive rates were 93.6%,95;7%with IFAT and 89.4%,93.7%with IEST,respectively.The results showed no significantly statistic difference(P>0.05).The false positive rate was 0.When sera from 34 cases with schistosomiasis,35 cases with clonorchiasis and 30 cases with tuberculosis were examind by IFAT and IEST, cross-reactions were 14.7%,5.7%,0 and 14.7%,8.6%,0 with IFAT,and 5.9%,0,0 and 8.8%,5.7%,0 with IEST,respectively. The results showed that both IFAT and IEST are useful mothods for serological diagnosis of paragonimiasis and adult,adolescent antigen on the epidermis, gut,excretory bladder was well demonstrated,while IEST might be more suitable in field surveys.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1995年第3期33-35,共3页
Chinese Journal of Zoonoses
基金
江苏省科学基金
关键词
成虫童虫
并殖吸虫病
间接荧光抗体
Frozen sections of the adult and adolescent worm Indirect fluorescent antibody test(IEAT)
Immunoenzymatic staining technique(IEST)
Paragonimiasis
