摘要
白细胞介素３（ＩＬ－３）是一种具有很强造血调控作用的细胞因子，本实验观察了成纤维细胞介导的ＩＬ－３基因疗法对大剂量环磷酰胺体内注射后造血功能损伤小鼠的恢复作用。结果表明：接受ＩＬ－３基因疗法的实验小鼠外周血白细胞和血小板数量降低程度减弱，回升速度加快，其脾脏和骨髓ＣＦＵ－ＧＭ、ＣＦＵ－Ｅ、ＣＦＵ－ＭＫ、ＣＦＵ－Ｓ水平显著地高于对照小鼠，可见成纤维细胞介导的ＩＬ－３基因疗法可显著地降低大剂量化疗后造血损伤程度，并能显著地促进受损的造血功能尽快恢复，提示若将ＩＬ－３基因疗法与大剂量化疗联合应用将提高肿瘤治疗的效果。
IL-3 acts as an important hematopoietic factor。In this study, the effect of IL-3 gene therapywas investigated on the recovery of murine hematopoietic suppression induced by high dose of cyclophaspho-mide(Cy).Our results showed IL-3 gene therapy could slow down the Cy-induced decreasing of pheripheralWBCs and platelets,and accerelate their recovery, elevate the CFU-GM, CFU-E, CFU-MK,CFU-S derivedfrom the splenocytes and bone marrow cells. The data demonstrated that fibroblast-mediated IL-3 gene ther-apy could significantly reduce the hematopoietlc damage resulted from chemotherapy to less extent,and ac-cerelate hematopoietic recovery from suppression,suggesting that the combination of IL-3 gene therapy withhigh dose of chemotherapy will achieve better therapeutic effect of cancer.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1995年第4期223-226,共4页
Immunological Journal
基金
国家自然科学基金
优秀中青年人才专项基金
关键词
白细胞介素3
基因治疗
成纤维细胞
药物疗法
Interleukin-3,Gene therapy, Fibroblasts,Chemotherapy,Hematopoietic recocery
