摘要
选择１９名老年人及１０名青年人为对象，研究了中药皂甙Ｒｇ１对其淋巴细胞应答增殖能力、表型及细胞膜流动性的作用。结果表明，老年人淋巴细胞对ＰＨＡ（植物血凝素）的应答能力比青年人低，用ＭＴＴ（噻唑蓝）法测定的ＯＤ（光密度）值分别为０．１５０８±０．０８１３５和０．１８２±０．０５；Ｒｇ１对老年人的应答能力有明显刺激作用，对青年人则否，说明Ｒｇ１的作用取决于ＰＨＡ刺激后淋巴细胞既定活化程度。并且证明Ｒｇ１对淋巴细胞的增殖为剂量依赖性双向调节。当ＰＨＡ为５μｇ／ｍｌ及Ｒｇ１为１μｇ／ｍｌ时可得最佳协同效应。用间接免疫荧光方法测检结果显示，Ｒｇ１明显增高ＣＤ（２５）、ＣＤ（４５）ＲＡ、ＣＤ（４５）ＲＯ各表型阳性细胞数，ＣＤ（４５）ＲＡ／ＣＤ（４５）ＲＯ比例随龄下降，青年人为０．９１．老年人为０．６６，Ｒｇ１对青年人、老年人淋巴细胞膜流动性都有显著的增强作用。作者讨论了老年人淋巴细胞功能降低及Ｒｇ１作用的机理。
Ninteen aged persons and 10 young adults were selected to study the effect of Rg1, a saponin from Chinese Medicinal Herb, on the proliferation, phenotype and membrane fluidity of their lymphocytes. The results showed that the response ability of aged lymphocytes to PHA is lower than that of young adults. the OD avlue detected with MTT method is 0. 1508+0. 08135 and 0.182+0. 05, respectively. Rg1 significantly increased the response of aged lymphocytes, whereas, an inhibilatory effect of Rg1 on the lymphocytes of young persons. It suggested that the action of Rg1 is depends on the definited activity after stimulation with PHA. A dose-denpendent bidirectional regulatory effect and Rg1 was presented at opeimal concentration of PHA(5μg/ml) and Rg1(1μg/ml) where the best synergetic efficacy occurred. Rg1 could markedly enhance the phenotypic expression of CD25, CD45RA and CD45RO on lymphocytes. The percentage of positive cell bearing different phenotype demonstrated by indirect immunofluorescence was elevated. The ratio of CD45RA/CD45RO was decreased during aging, that is 0. 91 for young people and 0. 66 for aged. Rg1 clearly accelerate the membrane fluidity of lymphocyte of both young and aged. The mechanism of the decline of lymphocyte function in the elderly and of the action of Rg1 were discussed.
出处
《中国老年学杂志》
CAS
CSCD
1995年第1期14-17,共4页
Chinese Journal of Gerontology
基金
国际老年学学会资助
