摘要
番泻甙、大黄多糖和大黄素对脑细胞内游离钙浓度的影响林秀珍，靳珠华（天津医科大学药理研究室３０００７０）近年来有关大黄及其有效成分的药理研究和临床应用有较大的进展［１，２］。但大黄有效成分番泻甙（ｓｅｎｎｏｓｉｄｅ，ＳＥＮ），大黄多糖（ｐｏｌｙｓａｃｃ...
Free intracellular Ca2+([Ca2+]i) levels in rat
brain were measured by Ca2+ sensitive fluorescent indicator
Fura-2/Am and the effects of sennoside(SEN),polysaccharides fromRheum
palmatum(RPP)and emodin(EMD)were studied.ResuIts showed that the
resting[Ca2+]ilevel in the brain cells was l76. 25 ± 44.67
nmol·L-1(n=10)in Ca2+-free Hank’s solutioncontaining EGTA 0,5
mmol·L-1.After adding CaCl2(2 mmol·L-1)and KCI(120 mmol·L-1)to the
brain cells suspension sequentially,the free[Ca2+]i levels were
obviously elevated(P<0.01)compared with that of resting level.The
brain cells were pretreated with SEN( 0.07,0.37mmol.L-1) for 10 min。
In the resting or using the above doses of CaCl2 and KCI,the[Ca2+]i
wereobviously decreased compared with the control groups(P<0.01)at
the same conditions.RPP dose-dependently decreased the [Ca2+]i.On the
contrary,when brain cells were pretreated with EMD(0.037 mmol·L-1)
for 10 min, the[Ca2+]i were obviously increased compared with the
controlgroups(P<0.01)at the same conditions.The results showed that
EMD could not only promote therelease of intracellular Ca2+ but also
the influx of extracellular Ca2+, The opposite effects of
thedifferent active components of rhubarb on the[Ca2+]i levels
suggest that rhuharb may have differentkinds of regulatory functions
on brain cells。
出处
《药学学报》
CAS
CSCD
北大核心
1995年第4期307-310,共4页
Acta Pharmaceutica Sinica
基金
国家自然科学基金
关键词
大黄
番泻甙
钙
实验药理
sennoside
Rhuharb
polysaccharide
EmOdin
Fura-2/AmThe project is supported by NationaI
Nature Science Foundation of China
