摘要
钙离子对４－氨基吡啶诱发肥大细胞释放组胺作用的影响徐建华，王佩，陈志刚，张剑 （浙江医科大学药学系药理学教研室杭州３１０００６）４－氨基吡啶（４－ａｍｉｎｏｐｙｒｉｄｉｎｅ，４－ＡＰ）是一种钾通道阻滞剂，它能促进神经末梢释放多种递质［１，２］，促进β...
Aminopyridine(4-AP) has been shown to induce
histamine release fromisolated peritoneal mast cells(PMC)in mice and
rats. In the presence of extracellular calcium atnormal level(0.9
mmol·L-1)histamine release induced by 4-AP(13. 6 mmol·L-1) from
mice PMCwas 33.0%±4.6%,while at low calcium concentration (0.5
mmol·L-1) and in calcium-freemedium this parameter decreased to
25.5%±4.2%and 16.3%±3.7%respectively.Histaminerelease in response
to 4-AP(10 mmol·L-1)from rat PMC was 39.1%±6.7%(0.9
mmol·L-1calcium),while at low calcium concentration(0. 5 mmol·L-1)
and in calcium-free medium thisparameter decreased to 29.3%±4.7%and
20.2%±2.9% respectively. Results of statisticalanalysis indicate
that 4-AP induced histamine release is related to Ca2+ concentration.
When rat PMCwere preincubated in calcium-free medium with EDTA 0.1
mmol·L-1 for 180 min 4-AP inducedhistamine release was 13.8%±1.6%.
This shows that 4-AP also elicited mobilization of endogenous
calcium stores in mast cells.The mechanism of 4-AP induced histamine
release was discussed.
出处
《药学学报》
CAS
CSCD
北大核心
1995年第3期235-238,共4页
Acta Pharmaceutica Sinica
基金
国家自然科学基金
