摘要
以番红花整个小球茎作外植体，培养在６８Ａ２～３ｍｇ／Ｌ（单位下同）和ＮＡＡ０．２～０．３的ＭＳ培养基上，出芽快而多．将无菌芽去尖接种到６ＢＡ４的培养基上，并加进少量ＮＡＡ，对丛芽诱导有利．愈伤组织和芽接种到６ＢＡ０．５和ＮＡＡ０．２的ＭＳ培养基上，均可长出小球茎．将带芽的小球茎接种到１ＢＡ１的１／２ＭＳ培养基上，可得到９６％的生根率．
As the whole little corms of Crocus sativus L. are taken as explants and cultured on MS mediumcontaining 6BA2～3mg/L and NAA 0.2～0. 3 mg/L, a lot of buds are induced quickly. Bud clustersinduction is enhanced, when aseptic buds with tip cutted are inoculated on to MS 6BA 4mg/L culturemedium with little NAA. If cailuses and buds are inoculated on to MS 6BA culfure medium with little-NAA. If calluses and buds are inoculated on to MS 6BA 0.5mg/L, NAA 0.2mg/L medium, bothof them can grow into little corms, When little corms with buds are transferred on to 1/2 MS mediumwith LBA1 mg/L, a 96 percent rate of rhizogenesis is obtained.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
1995年第2期183-186,共4页
Journal of Southwest China Normal University(Natural Science Edition)
基金
重庆市科委药用植物组织培养课题
关键词
番红花
组织培养
丛芽分化
中草药
Crocus sativus
tissue cultuves
formation of corm
bud differentiation
