摘要
以球茎切块为外植体,MS为基本培养基,0．5 mg·L^(-1)6-BA和2 mg·L^(-1)2,4-D为激素,在(20±2)℃,黑暗环境下培养,可以获得98%的愈伤组织诱导率．愈伤转入MS+5．0 mg·L^(-1)6-BA+5．0mg·L^(-1)NAA培养基中,可诱导丛生芽的产生,将丛生芽切成单个不定芽后转移到MS+4．0 mg·L^(-1)6-BA+0．5 mg·L^(-1)NAA的培养基中,光照条件下可诱导新生小球茎的产生．
Corms of saffron crocus （Crocus sativus） were used as explants and cultured in the dark on MS medium supplemented with 6-BA 0.5 mg/L and 2,4-D 2 mg/L. The callus induction rate was as high as 98 %. Tufty buds were obtained when the calli were transferred to an MS medium containing BA 5.0 mg/L and NAA 5.0 mg/L. The tufty buds were then separated into individual adventitious buds and cultured with illumination on a regeneration medium with 6-BA 4.0 mg/L and NAA 0.5 mg/L, and cormlets were successfully regenerated.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第1期68-72,共5页
Journal of Southwest China Normal University(Natural Science Edition)
