摘要
目的建立BSAS－ELISA技术以提高检测日本血吸虫循环抗原的敏感性。方法在夹心ELISA的基础上，引入高度放大的生物素一链霉亲和素系统（BSAS），建立BSAS－ELISA基本检测技术及其改良的二步法。结果应用单抗3D10以该技术的基本法与二步法检测感染兔血清，检出率可达90．00％；用单抗MG2以二步法经盲法检测，慢性血吸虫病患者的检出率达86．00％，3D10对正常人的假阳性率为4．76％，MGZ为33．33％。结论BSAS－ELISA检测日本血吸虫循环抗原可提高其敏感性。本实验所用二种单抗的特异性不甚理想，进一步提高敏感性及改进特异性的关键在于筛选出更理想的单抗。
Objective To develop BSAS-ELISA for improvement of sensitivity to detecting serum circulating antigen of Schistosoma japonicum. Methods On the basement of conventional sandwich ELISA, a high amplification , dtem, biotin-streptavidin system (BSAS), was utilized to develop a new basic method of the BSAS-ELISA and its modified two-step method. Results The positive rates of the basic and modified method in detecting infected rabbit sera with monoclonal antibody 3D10 were both 90.00%. In blind condition the positive rate for detecting chronic schistosomiasis patients sera by two-step method with monoc1onal antibody MG2, was 86. 00%. As to the specificity,the false positive rate in health control with 3D10 was 4- 76%, but was 33. 33% with MG2. Conclusious The new technique, BSAS-ELISA, may improve the sensitivity of detecting serum circulating antigen of Schistosoma jaPonicum. However, the specificity is not ideal. In order to improve its specificity, it is important to screen out some more ideal monoclonal antibodies.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
1999年第3期134-136,共3页
Chinese Journal of Schistosomiasis Control
关键词
日本血吸虫
循环抗原
ELISA
Schistosoma japonicum, Circulating antigen, Biotin, Streptavidin, ELISA, Detection
