摘要
采用免疫组化方法检测鸡免疫器官中CD4+、CD8+T细胞数量,探讨高温下清凉冲剂对机体细胞免疫机能的影响及其药理作用。108只农大3号35日龄公雏,随机分为3组,每组36只鸡,即常温对照组、高温对照组和高温用药组。用药组饲喂中药煎剂(浓度为1g/ml),用药量与饲料量比为1∶1000。在试验的第1、4、8、10天各组捕杀5只,分别采取胸腺、脾脏、法氏囊检测。结果高温下,用药组免疫器官中CD4+、CD8+T细胞数量均高于高温对照组,第8、10天差异极显著(P<0.01)。表明高温下清凉冲剂可以增加鸡免疫器官中CD4+、CD8+T细胞数量,有效地提高鸡细胞免疫水平。
Objective：To study the effects of Qingling electuary on the amounts of CD4^＋ and CD8^＋ T-cells under high temperature in chickens.Methods：108 Nongda Ⅲ 35-day-old male chickens were randomly divided into two control groups respectively under normal and bigh temperature,and treatment group under high temperature,which was given Qingliang electuary （0.1%） in water.Thymus,spleen,and bursa of Fabricius were collected from 5 chickens each group on 1^st,4^th,8^th and 10^th,respectively,to determine the average positive rates of CD4^＋ and CD8^＋ T-cells in the treatment group were higher than that of the control （P〈0.05 or P〈0.01）.Conclusion：Qingliang electuary could increase the amounts of CD4^＋ and CD8^＋ T-cells and improve the cellular immune function under high temperature.
出处
《中兽医医药杂志》
2006年第6期5-7,共3页
Journal of Traditional Chinese Veterinary Medicine
基金
北京市自然科学基金
北京市教委资助重点课题
编号KZ200310020007
北京市科委攻关课题(Y0705003041631)
