摘要
目的观察当归注射液对6－羟多巴胺（6－OHDA）致帕金森病（PD）大鼠模型的影响探讨当归注射液可能的作用机制。方法成年雄性Wistar大鼠采用随机数字表法分为模型组、小剂量当归注射液（12．5％）治疗组和大剂量当归注射液（25％）治疗组、空白对照组、生理盐水组，每组8只；采用6－羟多巴胺6-OHDA右侧中脑黑质注射制作大鼠帕金森病PD模型，模型成功后小剂量治疗组及大剂量治疗组每日1次腹腔注射当归注射液，生理盐水组注射等量生理盐水，空白对照组不做任何处理。旋转实验采用阿朴吗啡大鼠背部正中皮下注射后记录其每分钟旋转数，每周一次；治疗4周后采用SABC法行CD40、黑质酪氨酸羟化酶TH免疫组织化学染色。结果与模型组比较，大剂量当归注射液治疗组于治疗3周后旋转减慢差异统计学意义（P〈0．05），治疗4周后明显减慢差异统计学意义。治疗4周后，大剂量当归注射液治疗组右侧（注射侧）黑质TH、CD40阳性神经元数量较小剂量当归注射液治疗组及模型组明显增多差异统计学意义（P〈0．01）；空白对照组及生理盐水组右侧黑质未见CD40阳性小胶质细胞；模型组与治疗组右侧黑质CD40阳性小胶质细胞计数差异无统计学意义。结论当归注射液对PD大鼠有治疗作用，能明显减少黑质多巴胺神经元的丢失；当归注射液可能通过特定信号通路增加CD40在神经元的表达从而发挥其作用。
Objective To observe the therapeutic effect of Angelica injection in Parkinsonian rats and explore its possible mechanisms. Methods Rat models of Parkinson disease （PD） were established by 6-OHDA injection into the right substantia nigra pars compacta （SNc） and confirmed by weekly apomorphine-induced rotation test, and the rats with saline injection served as the saline control group （n=8）. After the second apomorphine test, the PD rats received daily intraperitoneal Angelica injection at low （12.5%, n=8） or high dose （25%, n=8）, or had only saline injection （PD model group, n=8）. Another 8 untreated rats were used as the blank control group. After 4 weeks of treatment, the rats were perfused with paraformaldehyde （4%） and the brains were taken for immunohistochemical detection of TH and CD40 expressions in the SNc using SABC method. Results Compared with the rats in the saline control and low-dose Angelica injection groups, those in the high-dose Angelica injection group showed significantly slowed rotation after a 3-week treatment （P〈0.05）, with further slowing down at 4 weeks （P〈0.01）. At the fourth week of treatment with high-dose Angelica injection, a significant increase in the numbers of PH- and CD40-positive neurons was found in the right SNc as compared with those in the low-dose Angelica injection group and the PD model group （/9〈0.01）. No CD40-positive microglia were found in the SNc in the saline control or the blank control groups, and the Angelica-treated groups and the PD model group showed no significant difference in the count of CD40-positive microglia in the SNc. Conclusion Angelica injection produces obvious therapeutic effect in Parkinsonian rats possibly by increasing CD40 expression in the neurons.
出处
《中华神经医学杂志》
CAS
CSCD
2008年第11期1151-1154,共4页
Chinese Journal of Neuromedicine
基金
湖北省卫生厅科研基金（JX3B23）
关键词
帕金森病
当归注射液
Parkinson disease
Angelica injection
