摘要
目的:建立同时测定精制桂枝茯苓胶囊中3个有效成分——丹皮酚、苦杏仁苷、芍药苷含量的HPLC分析方法。方法:C18柱,流动相乙腈-水(含1‰磷酸),阶梯洗脱,流速1mL·min-1;MWD检测器。结果:苦杏仁苷线性范围为12.87-102.94μg·mL-1,r=0.9999;芍药苷线性范围为24.84-198.7μg·mL-1,r=0.9999;丹皮酚线性范围12.57-100.56μg·mL-1,r=0.9999。3个化合物的精密度与重复性RSD均<1.5%。结论:该法精密度高,重复性好,又可降低质量检验成本,可用于精制桂枝茯苓胶囊的质量控制。
Objective： To establish an HPLC method for the simultaneous determination of three major bioactive components in Jingzhi Guizhi Fuling capsules namely laetrile, paeoniflorin and paeonol. Method： A LiChrospher C18 column（4.6mm×250mm,5μm）was used. The chromatography was carried out with a stepwise gradient programming. The mobile phase was acetonitrile-water （containing 0.1% phosphorous acid ） and the flow rate was 1.0 mL·min^-1. Result： The linear range of laetrile was 12.87-102.94μg·mL^-1, r =0.999 9, paeoniflorin 24.84-198.7μg·mL^-1, r=0.999 9 and paeonol 12.57-100.5μg·mL^-1, r=0.999 9. The method is accurate with variation less than 1.5% and recovery more than 95%. Conclusion： The method was successfully applied to analyze three major bioactive components in Jingzhi Guizhi Fuling capsules.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2005年第16期1252-1254,共3页
China Journal of Chinese Materia Medica
基金
国家高技术产业发展项目计划(计高技[2001]2170号)
关键词
精制桂枝茯苓胶囊
有效成分
HPLC
定量分析
Jingzhi Guizhi Fuling capsule
effective components
HPLC
quantitative analysis
